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Photomanipulation & Microdissection (LCM) - Scanner-Based Photomanipulation – UGA-42 Series
RAPP - Model UGA-42 Firefly - Point Scanning Device for Localized Photomanipulation
The UGA-42 Firefly is one of the scanner-based systems of our UGA-42 series for localized photomanipulation. It is the basic system of this product range and is especially designed to illuminate small spots or larger regions of interest (ROIs) with high power density. Thus, the UGA-42 Firefly is the perfect scanner solution for all applications where high laser intensity is required.
RAPP - Model UGA-42 GEO - Selectable Shape Scanning Device for Localized Photomanipulation
The UGA-42 Firefly is an integral component of the scanner-based UGA-42 series designed for precise photomanipulation applications requiring high laser intensity. This system is adept at illuminating small areas or larger regions with remarkable power density, making it suitable for diverse scientific and research needs. Its modular structure allows integration and upgrades with up to four separate laser ports and other modules from the UGA-42 series, including the UGA-42 Geo and UGA-42 Caliburn. The system supports compatibility with most major research microscopes, facilitating a tailored approach for specific applications. The UGA-42 Firefly operates with precision and speed, powered by SysCon software that offers extensive customization for regions of interest and is equipped with real-time control for swift and accurate laser beam movement. The device's adaptability is further enhanced through easy synchronization with other equipment via TTL ports, enabling comprehensive experimental setups.
Microscopy and Imaging - Multi-Photon Microscopy
RAPP - Model MOM - Movable Objective Microscope for Two-Photon Imaging
The Movable Objective Microscope (MOM) by Sutter Instrument is a sophisticated two-photon microscope designed for advanced imaging within living specimens. This system features a unique design allowing three-dimensional movement and rotation of objectives while keeping the specimen stationary, optimizing the imaging process. The MOM integrates two independent microscopes: a wide-field section equipped with an Olympus vertical illuminator for standard epifluorescence, and a two-photon section that guides excitation laser light for deep tissue imaging. It supports precise and smooth X, Y, and Z movements with minimal drift and high reproducibility, akin to Sutter's MP-285 micromanipulator. This allows efficient Z-stack and mosaic image acquisition without moving the stage. The flexible horizontal light path facilitates conversion between upright and inverted configurations, suitable for imaging various non-horizontal surfaces. The MOM remains adaptable to evolving scanning technologies, supporting multiple scanner types, and offers customizable detector paths for optimal photon capture. Compatible with ScanImage software, it ensures seamless integration with imaging hardware.
RAPP - Model DF-Scope - Multi-Photon Imaging Package
The DF-Scope is a specialized multi-photon imaging package developed for the Olympus BX51WI upright microscope, designed specifically for laboratories engaged in electrophysiology and epifluorescent imaging. This package enhances the microscope's capabilities by integrating components essential for multi-photon imaging, leveraging a Ti:Sapphire laser for optimal performance. Key features include the incorporation of elements from the MOM system such as resonant and galvo scan boxes, controllers, detector paths, and high-sensitivity photomultiplier tubes (PMTs). These components facilitate the capture of light emitted from the sample's focal volume, a critical aspect of non-linear excitation imaging. The DF-Scope is equipped with dual detector paths, one positioned above and one below the sample, to maximize light collection. This configuration is especially beneficial for thin samples, such as brain slices, by utilizing sub-stage detectors for enhanced signal acquisition, adaptable with various Olympus condensers.Illumination Systems - LED & Bulb Systems
RAPP - Model KSL2 - Single Wavelength LED Illumination System
The KSL2 LED illumination system offers a high power, single wavelength light source for applications requiring precise sample illumination. Its compact design is complemented by a separate controller, allowing for both manual and software controlled usage, with support for analogue or digital modulation. The KSL2 is optimized for fast modulation and offers flexibility by integrating into various microscope systems, including those utilizing large camera sensors such as sCMOS or AMCCD. Users can achieve specific excitation due to its small bandwidth and expand their setup to include multiple excitation wavelengths by employing the beam combination cube FC-70. Its integration with the SysCon software adds to its utility, facilitating easy incorporation into UGA-42 photomanipulation setups. The KSL2's versatility is further enhanced with TTL ports for digital and analog external triggering, ensuring precise control tailored to experimental needs. It stands out by not requiring warm-up time and operating without excessive heat production, crucial for both living and fixed sample studies.
