Spark Holland B.V. products

State-of-the-art injection technology is important, naturally. And rapid sample turn-around time is very helpful, of course. Certainly, large sample capacity is very convenient, no doubt. But this all becomes irrelevant if you have only the slightest doubts about the integrity of the samples that you injected! Yet, while injection technology and capacity have seen great advances over the last 30 years, measures to maintain and control sample integrity have had little attention from instrument manufacturers. Until now. The new INTEGRITY™ autosampler from Spark sets a new standard in sample care.

Our generic autosampler for HPLC and LC-MS using state-of-the-art injection technology with fast injection and wash cycles. Efficient, multi-solvent needle wash virtually eliminates carry-over. ALIAS™ handles well plates and sample vials, either open or sealed, and provides true 4°C sample cooling. Its compact, stackable design and unrivalled performance make the ALIAS™ autosampler the best fit for your HPLC system… and your UHPLC system!

The online SPE cartridge for Symbiosis™/ Prospekt-2 / ACE systems has been specially designed for online elution to the HPLC column. Dimensions are optimized to combine high extraction capacity with small elution volumes. Standard dimensions are 10 mm in length and 2 mm inner diameter, but 10 x 1 mm is also available. Naturally, the cartridge will resist typical HPLC system pressures (max 300 bar). All common SPE sorbents are available in the Prospekt cartridge format. Therefore, existing SPE methods can be transferred to the Prospekt without additional SPE method development.

Dried blood spot (DBS) sampling is an emerging technology for bioanalysis, especially useful in the clinical and pharmaceutical laboratory, offering easy sample collection, transport and storage. Our revolutionary DBS Autosampler maintains the integrity of the sample through automation, offering time and cost savings. Innovative patented Flow-through desorption technology (FTD™)* provides direct elution of DBS from cards, offering not only consistent quality, while eliminating the inconsistency of tedious manual disc punching or costly robotics, but also allowing the option of on-line clean-up and analyte separation by SPE prior to analysis in an automated workflow. Automation of the entire workflow for DBS analysis in minutes, providing maximum sensitivity without any manual intervention. With the potential for many other biological samples, the DBS Autosampler from Spark sets a new standard for analysis!

OEM is in our genes, our high-end LC system with the  SPH1299™ UHPLC pump and Integrity Autosampler are available for OEM. In fact it is one of the very few state-of-the-art high-pressure gradient UHPLC systems available on the OEM market. And we are not just offering a top-class LC systems! Changing industrial designs for OEM business has become second nature to us, the SPH1299™ UHPLC pump and Integrity autosampler has been designed for easy customization. We aim to be your partner at every stage – from customizing the industrial design to creating and integrating its driver in your software package. Our quality management complies with ISO 9001 and ISO 13485 and our products are REACH and RohS compliant. We have over 30 years of experience in the development and production of UHPLC instruments.

1240 bar (18000 psi). Up to 5 mL/min. High pressure gradient. Build in degasser. Ideal for HPLC, UHPLC and Core Shell. Injection volume from 1 µL to 1000 µL Low carry-over due to multiple wash solvents. Sample cooling. 2 high or low well plates or 48 vials. Optional Column oven. Upgradable to Symbiosis on-line SPE system. Solvent storage. Runs under AnalystTM version 1.4.1 and higher. Uses AnalystTM security settings. Uses AnalystTM Audit Trail settings. Records all LC information per chromatogram in the *.wiff file. Also controllable through Sparklink 4.10.

Almost every parameter defining a chromatographic separation is temperature dependent. Suffice it to say that temperature control provides an indispensable tool to optimize your HPLC or UHPLC assay. As you will easily recognize, retention times will be more constant with constant temperature, but varying the temperature can also be a great help to tune selectivity, improve peak shape and reduce analysis time. Plus, high temperatures reduce column back pressure significantly, allowing higher solvent flow rates, narrower columns or smaller particles. Clearly, such benefits will only happen if the temperature itself is stable and precise, and without temperature gradients in the oven or the column. And this is exactly what we had in mind when we designed the MISTRAL™ with its forced air oven, excellent temperature control and integrated solvent pre-heater.

Innovative pumping technology created Holland, now Spark Holland creates a novel pump. The SPH1299™ UHPLC pump is an advanced workhorse for robust, state-of-the-art UHPLC performance and beyond. Delivering ultra-precise UHPLC gradient flow up to 18,850 psi (1300 bar). Featuring truly automatic compressibility compensation, real self-priming and clearing plus simple maintenance, this pump will turn users into friends. Robust proven concept guarantees reliable performance. True automatic self-priming. Accurate flow rate – what you set is what you get. Easy and economic user maintenance.

Traditional volumetric dispensers are designed for pipetting reagents or samples into vials or wells at ambient pressure or a few Bar at most. Online volumetric reagent delivery however, may require the ability to introduce reagents into a system with a high working pressure. The HPD™ was originally designed by Spark for SPE solvent delivery in on-line SPE-LC systems, but other applications are easy to imagine. Think of pre-column reagent addition for online derivatization in HPLC, post-column reagent addition for detection enhancement, etc.

In 2018, Spark Holland and Axel Semrau have signed a global commercial agreement for the distribution of Spark Symbiosis legacy. Axel Semrau® offers different online SPE front end solutions based on Spark technology. All our CHRONECT Symbiosis solutions are UHPLC systems combined with an online SPE front end and the robust and reliable PAL autosampler with flexible options for sample preparation.