Biochemical Separations and Tutorials Brochure (PDF 58 KB)

BIOCHEMICALSEPARATIONSThe State University of New Jersey - Rutgers Campus at New BrunswickA Two - DayLecture CourseThe Center for Research and Education inBioluminescence and Biotechnology(CREBB)presents:An Introduction to Laboratory Techniquesin Biotechnologyhttp://www.rci.rutgers.edu/~crebb/biosep.htmlFebruary 15-16, 2007April 26-27, 2007August 2-3, 2007February 2-4, 2007May 4-6, 2007August 10-12, 2007TUTORIAL INPROTEINPURIFICATION3-Day Hands-On Instruction in DownstreamProcessing Techniqueshttp://www.rci.rutgers.edu/~crebb/Tutorials.htmlDr. Ward is a noted teacher and seminar leaderwith more than 20 years of experience with adultprofessional audiences. He created "BiochemicalSeparations" in 1984 and the course has been run-ning continuously ever since. Dr. Ward special-izes in the chemical and physical properties of the green-fluorescent proteinand other proteins involved in marine bioluminescence. He is published in 112books, book chapters, journal articles and abstracts. Dr. Ward has co-authored(with Catherine Thomson, Ph.D.) "A Guide to Green-Fluorescent Protein:Applications in Cell Biology and Drug Discovery" for D&MD Publications.This resource-oriented practical guide presents an overview of the most impor-tant features and technological applications of Green-Fluorescent Protein (GFP)and its variant forms, and illustrates how GFP is currently being used in thebiopharmaceutical industry. He has also authored "Biochemical and PhysicalProperties of Green-Fluorescent Proteins", a chapter in "Green-FluorescentProtein: Properties, Applications, and Protocols, 2nd Edition", Chalfie andKain (eds.), Wiley-Liss, Inc. 2006.Faculty CoordinatorThe Center for Research and Education inBioluminescence and Biotechnology (CREBB)The Center offers a series of continuing education workshops each year featur-ing nationally renowned presenters. The Center for Research and Education inBioluminescence and Biotechnology (CREBB) is a component of Rutgers Uni-versity, School of Environmental & Biological Sciences (SEBS). The CREBBmission is to perform basic research on bioluminescence and to utilize biolu-minescence (especially the Green Fluorescent Protein) as a tool to educate thescientific and industrial communities in the field of biotechnology.Dr. William W. Ward,Associate Professorof BiochemistrySchool of Environmentaland Biological Sciences,Rutgers Universityand Director of C.R.E.B.BThis is an introductory-level course designed to acquaint participants withthe wide range of modern techniques available for separating and purifyingbiomolecules. The fundamentals of each technique will be presented, in-cluding practical examples; however, sufficient theoretical background willbe provided to enable the participants to understand how each techniquefunctions. Emphasis will be placed on techniques used in protein isolationand purification - both from native and recombinant sources. The coursewill be particularly valuable to those who are beginning work in biochemis-try and biotechnology laboratories and who wish to broaden their back-ground in separation procedures used in biochemistry. “Biochemical Sepa-rations” may be useful for persons getting back into the laboratory afterseveral years, for those between jobs, and for scientists whose job descrip-tion now requires familiarity with separation techniques used in Down-stream Processing.After completing this course, participants will be well prepared for the5 1/2 day laboratory course "Protein Purification: Principles and Prac-tice". Course Website: http://www.rci.rutgers.edu/~crebb/protein.htmlCourse DescriptionUpcoming Biotech Course offered by CREBBProtein Purification:Principles and Practice(5½ days, laboratory/lecture/discussion)This, our centerpiece course, has been offered morethan 40 times over the past 17 years to a total audi-ence in excess of 1,200. Participants isolate GFP fromjellyfish or transformed E.coli cells and purify the pro-tein to homogeneity in a series of chromatographicsteps including: gel filtration, ion exchange, hydropho-bic interaction, and size exclusion HPLC. Subsequentelectrophoretic analyses include SDS PAGE, nativePAGE, IEF, preparative PAGE and Western blotting.The course concludes with an interactive workshopduring which participants work in groups to deducethe structure of the GFP chromopeptide.Course Cost: $2,695January 7-12, 2007March 11-16, 2007June 3-8, 2007July 8-13, 2007July 22-27, 2007(all Courses run Sunday through Friday)Course Website:http://www.rci.rutgers.edu/~crebb/protein.htmlNote: If you register three weeks prior to the start of thecourse you pay only $2,195.A discounted fee of $1,995 per person is available if two ormore registrants from the same company register.There is also a discounted fee of $1,395 for attendees fromacademia.The tuition fee includes continental breakfasts, lunches,coffee breaks and three dinners, along with all coursematerials and hand-outs.Tuition Fees MUST BE PAID at least 3 weeks prior to thestart of the course to be eligible for the discounted rates.Course OutlineDay 1 8:00am-5:00pm Introduction and Continental Breakfast INTRODUCTION TO THE PROPERTIES OFBIOMOLECULESChemical and physical properties of proteins, nucleic acids,carbohydrates, lipids, and other biomolecules. Generalstrategies for molecular separations will be introduced. PREPARATIVE METHODSSurvey of batch methods (salting out, isoelectric precipitation,and batch adsorption), dialysis, filtration, and ultrafiltration.Selected methods used in enzyme purification will bepresented in detail.Lunch BASICS OF LIQUID CHROMATOGRAPHYOF BIOMOLECULESPrinciples of liquid chromatography. Emphasis will be placedon modern methods of low pressure liquid chromatographyincluding gel filtration chromatography. Elementary chro-matographic theory will be introduced. ION EXCHANGE AND HYDROPHOBICINTERACTION CHROMATOGRAPHYA survey of ion exchange and hydrophobic (reverse phase)separation methods for proteins, nucleic acids and smallermolecules. Examples will include both high pressure and lowpressure methods.BiochemicalDay 28:00am-5:00pmContinental Breakfast AFFINITY CHROMATOGRAPHYAn introduction to this powerful method for purifyingenzymes, antibodies, nucleic acids, and polysaccharides.Examples will include immobilized metal ion affinity chroma-tography, and affinity chromatography of enzymes withimmobilized substrate analogues. Separation of recombinantproteins will be emphasized. CONTEMPORARY PRACTICE OF LIQUIDCHROMATOGRAPHY (HPLC)An overview of the basic operating principles of HPLC with acomparison to other available tools. Special emphasis will beplaced on the role of modern liquid chromatography as aproblem-solving tool for bioanalytical research.Lunch New for 2007 """"""""""TUTORIALS IN PROTEIN PURIFICATION(3 days, hands-on instruction in Downstream Processing)February 2-4, 2007 (Friday - Sunday)May 4-6, 2007 (Friday - Sunday)August 10-12, 2007 (Friday - Sunday)In an intimate, small group laboratory setting, learn the details of proteinpurification from an expert. Join Professor William Ward as he leads youthrough the step-by-step purification of a model protein, hands-on fromstart to finish. Under his constant supervision, you will prepare a crudeextract, administer several batch purification steps, and then achievepurity in a series of high resolution chromatography steps. You will thenjudge purity of the final product in comparison with your crude extractby calculating specific activity and by analyzing your product by sizeexclusion HPLC and/or SDS gel electrophoresis.As you assist in setting up equipment, carrying out experiments, andanalyzing results, you will have many opportunities to benefit fromProfessor Ward’s extensive experience as a long-time, practicing proteinbiochemist.So that your specific questions can be properly addressed in one-on-onedialogue, registration will be limited to just 8 participants.Course sessions run three days, Friday thru Sunday with lunch providedon Saturday and Sunday as follows:Friday - 2 pm to 6 pmSaturday - 9 am to 5 pmSunday - 10 am to 3 pmCourse Cost: $995Course OutlineSeparations FUNDAMENTALS OF ELECTROPHORESISBasic principles of electrophoresis and survey of electrophoreticmethods. Emphasis will be placed on polyacrylamide gelelectrophoresis of proteins, molecular weight estimation by SDSPAGE, and isoelectric focusing. SEPARATION TECHNIQUES IN PROTEINAND DNA SEQUENCINGUse of HPLC reverse phase chromatography in the sequencing ofproteins by automated Edman degradation. Discussion ofelectrophoresis of nucleic acids as it applies to the sequencing ofDNA.Course LocationThese courses will be held on the School of Environmental andBiological Sciences Campus, New Brunswick, NJ. A map with writtendirections to the course site will be included with your confirmation ofregistration. If you do not receive your confirmation ten days beforethe course starts, please call our Registration Desk at (732) 932-9763ext. 225 or 216.RefundsYou may withdraw from these courses with a full refund (less a $100processing fee) provided our office is notified at least five (5) fullbusiness days prior to the start of the course. Beyond that time, youmay be responsible for the full tuition fee if you register but do notattend. Substitutions are encouraged.CREBB reserves the right to cancel these courses due to insufficientenrollment.Registration InformationThe tuition for each of these courses is $995. Register three weeksprior to the start of the course and pay only $895. A discounted fee of$850 per person is available if two or more registrants from the samecompany register. The tuition fee includes continental breakfasts,lunches, and coffee breaks along with all course materials and hand-outs.Fees MUST BE PAID three (3) weeks prior to the start of the course tobe eligible for the discounted rates.""""" By Phone9:00 a.m. – 4:00 p.m., Monday – FridayRegistration Desk: (732) 932-9763 ext. 225 or 216##### By MailSend check or money order (payable to Rutgers, the State University)to:CREBB Registration DeskRutgers UniversitySchool of Environmental and Biological SciencesDepartment of Biochemistry and Microbiology76 Lipman DriveNew Brunswick, NJ 08901-8525By Credit CardVisa or Master CardCall Registration Desk (732) 932-9763ext. 225 or 216 By Fax(732) 932-3633, 24 hoursPlease fax a copy of check or money order with fax registration." Via the InternetBiochemical Separationshttp://www.rci.rutgers.edu/~crebb/RegisterBiosep.htmTutorials in Protein Purificationhttp://www.rci.rutgers.edu/~crebb/RegisterTutorials.htmlWays to RegisterFor information on travel and lodging, please contact:Professor William W. Ward at (732) 932-9763 ext. 216(email: crebb@rci.rutgers.edu), Gloria Kierniesky at ext. 225 (email:kierniesky@aesop.rutgers.edu), or phone the Rutgers Information Service at(732) 932-INFO.Please visit the following Rutgers web site for information on discounted traveland lodging: http://www.rutravel.rutgers.edu/discountprograms.htmlTuition Fee:$895 (Early Registration)$850 (Per person forMultiple Early Registration of 2 ormore attendees from the same company)$995 (Regular Registration)NOTE: Fees MUST BE PAID3 weeks prior to the start of thecourse for all discounted rates.$Check or Money Orderenclosed in the amount of$______________$Visa or MasterCard# _________________________Exp.Date:____________NameFirst Name For NametagEmployerJob TitleMailing AddressWork PhoneHome PhoneFax No.E-mail AddressRutgers UniversitySchool of Environmental and Biological Sciences(CREBB)Department of Biochemistry and Microbiology76 Lipman DriveNew Brunswick, NJ 08901-8525Biochemical Separations%February 15-16, 2007%April 26-27, 2007%August 2-3, 2007Tutorials in Protein Purification%February 2-4, 2007%May 4-6, 2007%August 10-12, 2007Nonprofit OrganizationU.S. Postage PaidNew Brunswick, NJPermit # 1296CREBB, Rutgers UniversitySchool of Environmental and Biological SciencesDepartment of Biochemistry and Microbiology76 Lipman DriveNew Brunswick, NJ 08901-8525BIOCHEMICALSEPARATIONS &TUTORIALS INPROTEIN PURIFICATION