Green Gene Screen Product Sheet

Protein Mini-Prep Thepinstfold conith most proteins of interest, the GGS protein mini-prep preserves biolog solubility FP is being used to monitor metastasis, stem cell colonization of damaged tissues, optimization of gene e “GREEN GENE SCREEN” is a novel protein mini-prep that rapidly and efficiently xtracts, purifies, and concentrates selected proteins from a variety of natural and recombinant sources, including cells and tissues of mammalian origin and whole, unlysed bacterial cells. Originally developed for rapid extraction and accurate quantitation of recombinant Aequorea GFP, the GGS kit can also be used successfully with many other proteins of interest, including proteases, Cytochrome c, Protein A, Protein G, IgG, IgY, eroxidases, and many other proteins. The GGS kit contains all the materials and procedural ructions necessary to achieve instant extraction, up to 100-fold purification, and up to 50-centration. When applied to the quantitation of GFP in cells and tissues, the GGS kit virtually eliminates turbidity, light scatter, and autofluorescence that normally complicate GFP measurements. Wwhile accomplishing extraction, purification, and concentration in just a matter of minutes. The GGS protein mini-prep comes complete with procedural instructions that enable the user to optimize, by a simple dilution procedure, purification of the protein of interest. For those who wish to quantitate GFP, appropriate GFP standards are included as well as freeze dried E. coli cells expressing recombinant GFP. If you wish to purify a protein other than GFP, the GGS kit allows you to practice the technique on transformed E. coli cells included in the kit. A UV penlight that effectively excites GFP is included for your convenience. With the exception of a standard microfuge, no specialized equipment is required. At the completion of the mini-prep treatment, the now highly purified and concentrated samples are ready for activity assay and for analysis by SDS gel electrophoresis, HPLC, Western blotting, and other methods. ical activity and Ginduction, fusion protein production, down stream optimization of all sorts, drug discovery, etc. Many industrial labs use multimillion dollar confocal microscopes to see GFP in cells, but microscopy is not a good way to quantitate GFP levels. Others use fluorescence activated cell sorting, but this technique is also very expensive, is limited to cell suspensions, and is hard to quantitate. A simple, inexpensive, self-contained kit that facilitates spectrophotometric or fluorometric quantitation of GFP has not been commercially available until now. We announce for the first time, the development of such a kit, called the Green Gene Screen. This kit quickly and efficiently releases GFP from cells and tissues, purifies that GFP up to 100-fold, and concentrates the GFP by 20 to 50-fold. It can be used on free GFP or on a variety of GFP fusion proteins. A major advantage of our GGS kit is its ability to quickly remove all optically interfering substances and scattering particles from even the most crude extracts and cell suspensions. By removing all the optically interfering substances and turbidity, the Green Gene Screen kit makes possible an accurate, quantitative measure of GFP content. Fig. 1. Using the GGS kit, a highly Fig. 2. Wavelength scan of diluted, purified GFP disk (on the right) is obtained from whole unlysed E.coli cells expressing GFP (on the left) in one simple procedure. unlysed E.coli cells expressing GFP. Fig. 3. Wavelength scan of resuspended GFP disk after GGS prep. A 112-fold purification and 81% recovery of GFP was achieved.