LSC consumables

LSCConsumablesLSC cocktailsCocktails for measuring RadonOxidizer/Pyrolyzer suppliesSolubilizersLSC vials? ProFlow G + - General PurposeGeneral purpose, non-gelling , flow cocktail with low viscosity(allowing easy mixing and pumping) for use with diluteeluents and organic/aqueous gradients.? ProFlow P + - Phosphate GradientsGeneral purpose non-gelling flow cocktail with low viscosity(allowing easy mixing and pumping) for use with 0 to 2Mammonium phosphate gradients.The ProSafe+ range is an improved version of the original ProSafe range. The solvent base hasbeen changed from PXE to DIN and this has necessitated a minor change to the formulations.Overall the changes have produced cocktails with even higher Tritium efficiencies, bettersample holding capacities and a guaranteed compatibility with commercially available quenchsets.ProSafe+ cocktails are high flashpoint, 100% biodegradable, high performance LSC cocktails.They are designed to be in accordance with the EC Directive 2003/53/EEC. ProSafe+ cocktailsdo not contain any Nonyl Phenol Ethoxylates (NPEs) or other Alkyl Phenol Ethoxylates (APEs)which produce endocrine disrupting metabolites upon biodegradation and are thus suitable fordrain disposal, depending on radionuclide content of the counting samples and local radiationprotection regulations.Different ProSafe+ cocktails are available to cover a wide range of sample type counting.? ProSafe+ FCHigh efficiency cocktail for use with wet and dry filtersand lower volume (1 – 2 mL) of aqueous samples.? ProSafe HC +High capacity cocktail accepting 10 mL of many dilutesamples and lesser amounts of more concentratedsamples in 10 mL cocktail.? ProSafe +High performance cocktail accepting 4-6 mL of manydilute samples and lesser amounts of moreconcentrated samples in 10 mL of cocktail.? ProSafe TS +Specialist cocktail for use with solubilised (digested)samples originating from both organic and aqueoussolubilizers.ProSafe+ CocktailsFigure 1:A-Before degradationB- After treatmentA B0,00 1,00 2,00 3,00 4,00 5,00 6,00 7,00 8,00 9,00 10,00DI Water0.1M PBS0.15M NaCl0.05M Tris-HCl0.2M NaH2PO40.1M NaOHUrine1.0M H3PO44.0M H3PO42.0M HNO31.0M HCl4.0M HClMaximum volume (mL) of aqueous solution to be mixed homogeneously with 10mL LSC cocktailUptake by 10mL scintillation cocktails of various aqueous phasesProSafe+ ProSafe HC+Figure 2: Uptake of various samples in 10 mL of ProSafe+ cocktailsStarGelml water added to 10.0 ml Star Gel17°C 21°C0.00 ml Clear Clear0.50 ml Clear Clear1.00 ml Clear Clear1.50 ml Clear Clear1.60 ml Unstable Unstable1.90 ml Unstable Unstable2.00 ml Gel Gel2.40 ml Gel Gel2.50 ml White gel White gel>2.50 ml Unstable UnstableStarGel is a classical high flashpoint DIN based, biodegradable LSC cocktailspecifically designed for suspension counting where the particulate matter isheld in the gel phase. It is odourless and compatible with plastic vials. Thecocktail shows very low quench with a tSIE of approximiately 590 and acounting efficiencies of ~94% for C-14 and ~48% for H-3 under theseconditions. It further shows a low background count rate of < 20 CPM in a 0 –18.6 keV window (H-3). StarGel forms a stable gel with small amounts of water(2.3 – 2.5 mL per 10 mL StarGel) at 17° - 21°C. The cocktail contains NPEs and isthus not drain disposable.Table 1: Water uptake of 10 mL Star Gel at varying temperatures ???? Gold StarHigh sample capacity, high counting efficiency cocktail foraqueous and organic samples. The Cocktail will accept 10mL of many dilute samples and lesser amounts of moreconcentrated samples in 10 mL of cocktail.GoldStar cocktails are classical high flashpoint DIN based, biodegradable LSCcocktails designed for high sample capacity and counting efficiency. GoldStarcocktails contain NPEs and are thus NOT drain disposable.GoldStar Cocktails? Gold FlowNon-gelling, low-viscosity, high counting efficiencycocktail for Methanol / Water and Acetonitrile / Watergradients.Figure 3: Uptake by 10mL GoldStar of various aqueoussolutions at 15 and 20°C.Figure 4: Uptake by 10mL GoldFlow of various aqueoussolutions.? GoldStar LT2 (Low Tritium @ Low Temperature)GoldStar LT2 is a high performance cocktail that will accept up to 12mL water in10mL of cocktail. It has especially been designed for high water and urinecapacity at various temperatures, including low temperatures, and high tritiumcounting efficiency, in order to allow its use, amongst others, for low leveltritium measurement.The cocktail can take up to 12mL of water samples with low matrix content(e.g. tap or deionised water) per 10 mL of cocktail at temperatures between10°C and 20°C. For matrix rich water samples like sea water the uptake shows avery strong temperature dependency with low temperatures resulting in veryhigh sample uptake (Fig. 5).Samples are stable even at 10°C and the cocktail shows no permeation through plastic vials, thusallowing extended-time counting which, in combination with its low background in an optimizedTritium window, allows obtaining very low detection limits. The cocktails can take up more than 3 mLof urine in 10mL of cocktail, nevertheless due to increasing quench with increasing urine volume itwas found that 2.5 – 3 mL of urine in 10 mL of cocktail gave optimum performance.Figure 6 compares the uptake of samples of various mineral acids and acid concentration in GoldstarLT2 and GoldStar; in general GoldStar LT2 allows working at higher acid concentrations than GoldStar.The performance of GoldStar LT2 in terms of background count rate, quench and MDA (minimumdetectable activity) obtained was compared to the performance of Ultima Gold LLT and Ultima GolduLLT (Fig. 3, tables 2 and 3); very similar results were found.Figure 6: Comparison of the capacity of 10mL of Gold Star and Gold Star LT2 LSC cocktails mixed with different aqueous solutions.Figure 5: Uptake of various types of water samples in 10 mLGoldStar LT2 at varying temperaturesLow Level Counting Data Conditions A1. TriCarb 2550 TR2. 10 ml DI water3. Low level count mode4. 0.5 – 4.5 keV window5. 300 minutes count time6. Temp 11°CGold Star LT2 Ultima Gold LLTcpm 2.9 2.5tSIE 285 285MDA 0.29 Bq/L 0.27 Bq/LLLD 1.53 Bq/L 1.43 Bq/LRed = Gold Star LT2Green = Ultima Gold uLLTLow Level Counting Data Conditions B1. Quantulus2. Channels 5-170 (optimised Tritium region)3. 10 ml DI water4. 600 mins count time5. Temp 18°CGold Star LT2 Ultima Gold uLLTcpm 0.84 ± 0.04 0.70 ± 0.03Figure 7: Counting spectra comparison depending on LSC used Table 3: Results obtained with low level counting data conditions B Table 2: Results obtained with low level counting data conditions A ???? GoldiSol - IPA based, Toluene and Methanol free non-toxic solubilizer.Solubilizes tissues, tissue homogenates, whole blood and high water content samples such asplasma, brain and polyacrylamide gels. Does not froth when peroxide is added to decolorize thetissue digest, resulting in simple and easy decolorisation.???? AquiGest - Aqueous based solubilizer (not classified as flammable or toxic).Can replace organic solvent based solubilizers for a wide range of samples while providingequivalent or even faster dissolution. Resulting tissue digests are in general less coloured thandigests obtained with organic solvent based solubilizers.SolubilizersRn CocktailsRadon cocktails are water immiscible LSC cocktails designed for the measurement of Radon in waterafter its extraction from the sample into the cocktail.???? RadonCount - Pseudocumene based cocktail with a flashpoint of approx. 49°C.???? ProScint Rn - Safer version of RadonCountbased on a combination of mineral oil and pseudocumene with a flashpoint of approx. 65°C. Capacities @20° C Gold Star Gold Star LT2 ProSafe+ ProSafe HC+0.1M citric acid 10.0 ml 0.75 - 10.0 ml 3.25 ml 7.5 ml0.1M ammonium citrate 10.0 ml 1.25 - 7.5 ml 2.75 ml 5.5 ml0.1M EDTA 10.0 ml 2.25 - 4.5 ml 3.40 ml 5.25 ml0.05M HNO3 10.0 ml 10.0 ml 3.75 ml 10.0 ml0.35M HNO3 10.0 ml 10.0 ml 5.75 ml 10.0 ml2M HNO3 4.25 ml 2.75 ml 2.75 ml 4.5 ml3M HNO3 3.25 ml 2.25 ml 2.25 ml 4.25 ml4M HNO3 2.75 ml 2.25 ml 2.50 ml 4.0 mlTable 4 shows the result of a series of experiments performed by James Thomson (MeridianBiotechnologies Ltd.) testing the maximum accepted volume of four liquid scintillation cocktails foraqueous solutions most commonly used for radionuclide elution from our extraction chromatographicresins.Table 4: Uptake of typical eluting agents in 10 mL of four different Meridian LSC cocktails at 20°CPlastic vialsMeridian plastic vials are manufactured from virgin High Density Linear Polyethylene (HDLP) ensuringuniformity of structure. Counting backgrounds are low (5-7 cpm in a 0-18.6 keV window) givingexcellent performance. Using HDLP ensures no high flash point cocktails, such as Gold Star and GoldStar LT2 permeate through the vial wall. Extended time testing show no loss of sample. Tests over amonth show no loss of sample.The vials are available in various sizes. For vials prepacked in trays, caps are delivered either screwedon the vial in in separate bags.Glass vialsMeridian Glass Vials are manufactured with low background quartz materials with minimal detectableimpurities. This ensures a performance benefits of low background (12-13 cpm in a 0-18.6 keVwindow) at low price. For user safety, the glass is annealed to prevent brittleness andminimize breakages. Vials are available in different packaging. Caps are packed separately inconvenient bags of 100 and have a flat surface for writing or labelling.Volume of vialsqty of vials -prepacked in trays of 100Caps20mL 500Highreflection20mL 500 Polycone8mL 1000 PolylLinedVolumeof vialsqtyPrepackedin traysIn boxes Caps20mL 1000 - yesPOPSCREWor PUSHIN20mL 500 100/tray -7 mL 2500 - yes8mL20001024-256/trayyes-POPSCREWVialsTable 5: Characteristics of plastic vials.Table 6: Characteristics of glass vials.???? CarbonTrap - High capacity carbon dioxide absorber (CO2 capacity: 4,8mM.mL-1) used to trap radioactive carbon dioxide produced in sampleoxidisers and pyrolysers.???? CarbonCount - Specific cocktail for the counting of radioactive carbondioxide captured in CarbonTrap.Oxidiser and Pyrolyser suppliesWe also provide•Specific chromatographic resins for the separation of actinides, strontium, nickel, lead…, •Ion exchange resins•Associated accessories•Technical assistance Do not hesitate to contact TrisKem International > contact@triskem.fr,and/or visit us at > www.triskem-international.comFor technical support, you may contact shappel@triskem.fr, abombard@triskem.frFor commercial request/offer, you may contact contact@triskem.frMeridian PerkinElmerProSafe+ Ultima Gold / Hi-Safe 2ProSafe HC+ / Gold Star Ultima Gold XR / Hi-Safe 3ProSafe FC+ Ultima Gold MV / Supermix / Filter-CountGold Star LT2 Ultima Gold AB / Ultima Gold LLT / Tri-SafeProSafe TS+ Hionic FluorProScint RnUltima Gold F / Scint Hi-Safe / High EfficiencyMineral Oil ScintillatorRadonCount Insta-Fluor Plus / OptiScint SafeStarGel InstaGelProSafe G+/Gold Flow Ultima-Flow M / Opti-Flow Safe IProSafe P+ Ultima-Flo AP / Ultima-Flo AFCarbonCount Permafluor E+ / Optisorb-SCarbonTrap Carbosorb E / Optisorb-1GoldiSol Soluene-350 / OptisolveAquiGest SOLVABLEHyamine hydroxide Hyamine hydroxideCocktail Equivalents ChartTRISKEM INTERNATIONALParc de Lormandière Bât. C – Rue Maryse Bastié – Campus de Ker Lann – 35170 Bruz – FranceTel +33 (0)2.99.05.00.09 – Fax +33 (0)2.99.05.07.27 – www.triskem-international.com – email : contact@triskem.frSAS au capital de 40.000 euros – SIRET 493 848 972 00011 – APE2059Z – TVA intra communautaire FR65 493 848 972 06/01/2012