NTK-MPSR - Standard Range Microplate Nitrate Test Kit - Brochure

This Nitrate test kit is based on the enzyme Nitrate Reductase (NaR), catalyzing the reduction of Nitrate to Nitrite using the natural electron donor NADH. The Nitrite reacts with color reagents (dyes) under acidic conditions to produce a visible color. All reaction steps occur in the microplate. The concentration of Nitrate in the original sample is determined by measuring absorbance with a microplate reader using a 540 nm filter (or a filter as close to 540 nm as possible), versus Nitrate concentration in Nitrate Standards. Nitrate can be determined in water samples and extracts of plant tissues, soils and foods. The test is designed to measure Nitrate in the range of 0.5 to 10 ppm Nitrate-N using a 96-well microplate. The Nitrate concentration can also be expressed as μM Nitrate, where range is 36 to 714 μM Nitrate, or ppm Nitrate, where range is 2.0 to 44ppm Nitrate. Nitrite can also be determined by omitting NaR and NADH from the test (see Determining Nitrite, page 4).