HPLC Columns Articles

  • The Clog Blog

    Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen remarks from customers regarding clogging as though it ...


    By Restek Corporation

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Separation of quercetin from a Ginkgo Biloba mother tincture using the PrepChrom C-700

    The aim of this application note was to isolate a flavonol, quercetin, from a Ginkgo Biloba mother tincture. The prepurification of the mother tincture was performed on a reversed phase flash column by the means of dry loading. The target compound was identified by its characteristic UV-VIS spectra and the retention time determined by running a standard. Subsequently, the purification of the ...


    By BÜCHI Labortechnik AG

  • Arsenic, antimony and chromium speciation using HPLC-ICP-MS in selected river ecosystems of Upper Silesia, Poland – a preliminary study and validation of methodology

    Upper Silesia is the most transformed by humans of the Polish territories. As a result of mining, urbanization and other industrial activities, irreversible changes have taken place in the environment. In this paper, the method of collecting, preserving, storing and preparing samples from four Silesian rivers was optimized. Silesian sediment and river water samples are a challenge for the ...


    By IWA Publishing

  • News on Air 2013, Volume 2: Products for Separation

    Supelco® has developed the most extensive line of special purpose columns designed for industry specific applications; including environmental and industrial hygiene. These columns are manufactured to deliver high resolution, great analyte response, low bleed and long column life; allowing analysts to achieve the analytical performance they require. In this issue, you will find easy-to-read ...


    By MilliporeSigma

  • USLC columns put the right tools in your LC method development toolbox

    Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection The most significant influence on chromatographic peak separation, or resolution, is column selectivity. Unfortunately, column selectivity is also the least understood and most underutilized parameter. To improve selectivity, method developers often concentrate on ...


    By Restek Corporation

  • 70-min amino acid analysis of physiological samples

    Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Post-column derivatization with Ninhydrin offers unmatched selectivity and reproducibility of the analysis for most challenging matrixes. Pinnacle PCX post-column derivatization system allows analysts to combine eluant gradient capabilities of modern HPLC instruments with column ...


    By Pickering Laboratories, Inc.

  • o-Phthalaldehyde Reagent

    Introduction Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions. At a pH>9 and ambient temperature, reaction is generally complete within 30 seconds. The products of this reaction, 1-alkyl-2-alkylthio-substituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm. The reagents ...


    By Pickering Laboratories, Inc.

  • 30-minute amino acid analysis of hydrolyzed samples

    Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. No other tech¬nique has been shown to match the reproducibility and selectivity of ion-exchange analysis with post-column Ninhydrin detection. Nor is there a chromatography technique that provides as much information; the 570/440 nm signal ratio for each amino acid is a ...


    By Pickering Laboratories, Inc.

  • Multi-residue mycotoxin analysis single run analysis of deoxynivalenol, aflatoxins, ochratoxin a, zearalenone and fumonisin by HPLC and post-column derivatization

    Although Aspergillus (Aflatoxins, Ochratoxin A) are generally associated with peanuts and Fusarium (Deoxynivalenol , Zearalenone) with wheat, these fungi and those that produce other toxins are not host selective and so can cross plant species. This situation is complicated by the fact that the microscopic mold may not be visible to the naked eye. Also, when infected grains are processed, any ...


    By Pickering Laboratories, Inc.

  • Manufacturers face challenges with pharmaceutical impurities

    Pharmaceutical manufacturers may face various challenges when attempting to meet the United States Pharmacopeia (USP) requirements for residual solvents (volatile organic chemicals).Residual solvents in pharmaceuticals are trace level impurities of volatile organic compounds in final products or excipients. In general, residual solvents originate from manufacturing processes related to the ...


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