HPLC Columns Articles

  • Purification of natural products - Case Study

    “Due to its versatility, the PrepChrom C-700 is a valuable instrument in our natural product isolation based research group, where preparative techniques such as column chromatography, flash chromatography and preparative HPLC are used on a daily basis.” Customer name Prof. Dr. Timo Niedermeyer Company / Institution University of ...


    By BÜCHI Labortechnik AG

  • What is electrochemical detection and what are its applications?

    Electrochemical detection (ECD) for HPLC or UHPLC / UPLC is an extremely selective and sensitive detection technique that is applied in several analyses such as the neurotransmitters dopamine, serotonin and noradrenalin, or analytes such as 5HIAA, catecholamines and metanephrines. ECD has an enormous linear dynamic range of more than 6 orders of magnitude. This means that concentrations can be ...


    By Verulam Scientific Ltd

  • Enhanced Purification of Capsaicins with the CombiFlash® EZ Prep

    Abstract In this application note, capsaicins are used as a model for compounds difficult to resolve with flash chromatography. Capsaicin compounds possess very similar structures making them difficult to resolve from each other. Although these compounds can be resolved on flash chromatography columns, such as the RediSep® Rf Gold C18, preparative high pressure liquid chromatography ...


    By Teledyne ISCO

  • Microfiltration: Rocker VF12 Kit Saves Time and Money

    Filtering organic solvents for applications such as HPLC buffer prep can be challenging in order to ensure chemical compatibility with filters (PVDF, Nylon, or PTFE), preserving longevity of an HPLC ...


    By Sterlitech Corporation

  • The Clog Blog

    Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen remarks from customers regarding clogging as though it ...


    By Restek Corporation

  • Top 16 for 2016 - Restek LC article makes the cut

    If you are an avid chromatographer you likely subscribe to LC/GC magazine or its popular electronic version, E-Separation Solutions. It is definitely the place to go for news and information related to all things Chromatography. Over the years Restek has had many an article published in LC/GC and, full disclosure, we advertise there as well. Who in the business doesn’t? It is the place ...


    By Restek Corporation

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Glyphosate and AMPA in drinking water

    Carcinogenic or not? The broad-spectrum herbicide glyphosate is used all over the world in agriculture. Alongside farming, the chemical is also used for weed-killing in domestic gardens and in public and private spaces kept free from «vegetal invasion», such as railway tracks. Glyphosate has been used since the 1970s in pesticides and was hitherto thought to be harmless at ...


    By Metrohm AG

  • Find out the principles of ELSD and the gas delivery solutions for ELSD from a nitrogen gas generator

    ELSD is one of the most common detectors used with HPLC and is preferred to other detection methods for analyses such as carbohydrate, natural sugars and polymer analysis as well as being used for a wide range of other applications.  As a mass-detector, ELSD has advantages over some other methods of detection such as UV detection that require analytes to contain chromophores and it is ...

  • Find out the principles of ELSD and the gas delivery solutions for ELSD from a nitrogen gas generator

    ELSD is one of the most common detectors used with HPLC and is preferred to other detection methods for analyses such as carbohydrate, natural sugars and polymer analysis as well as being used for a wide range of other applications.  As a mass-detector, ELSD has advantages over some other methods of detection such as UV detection that require analytes to contain chromophores and it is ...

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • High-throughput Microscale Bioanalysis is not a Paradox

    Question: When someone says microscale chromatography, what comes to mind? Increased sensitivity? Reduced sample volume? Enhanced MS sampling efficiency? Yes, yes, and yes! You might also say, “finicky,” “slow,” or “only one person in our group knows how to use it!” Maybe even, “there’s no way I can successfully transfer that method, so why ...


    By Waters Corporation

  • ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines

    The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work as ...


    By Waters Corporation

  • Separation of quercetin from a Ginkgo Biloba mother tincture using the PrepChrom C-700

    The aim of this application note was to isolate a flavonol, quercetin, from a Ginkgo Biloba mother tincture. The prepurification of the mother tincture was performed on a reversed phase flash column by the means of dry loading. The target compound was identified by its characteristic UV-VIS spectra and the retention time determined by running a standard. Subsequently, the purification of the ...


    By BÜCHI Labortechnik AG

  • Arsenic, antimony and chromium speciation using HPLC-ICP-MS in selected river ecosystems of Upper Silesia, Poland – a preliminary study and validation of methodology

    Upper Silesia is the most transformed by humans of the Polish territories. As a result of mining, urbanization and other industrial activities, irreversible changes have taken place in the environment. In this paper, the method of collecting, preserving, storing and preparing samples from four Silesian rivers was optimized. Silesian sediment and river water samples are a challenge for the ...


    By IWA Publishing

  • News on Air 2013, Volume 2: Products for Separation

    Supelco® has developed the most extensive line of special purpose columns designed for industry specific applications; including environmental and industrial hygiene. These columns are manufactured to deliver high resolution, great analyte response, low bleed and long column life; allowing analysts to achieve the analytical performance they require. In this issue, you will find easy-to-read ...


    By MilliporeSigma

  • News on Air 2013, Volume 1: Products for Sampling

    For decades, Sigma-Aldrich has been a key supplier of innovative & high technology air monitoring products for various areas of gas/air sampling. Enclosed, you will find an overview on new and interesting devices to improve or simplify your air sampling. Supelco and Sigma-Aldrich offer a range of active and passive air sampling products, in addition to various analytical chemistry tools ...


    By MilliporeSigma

  • Carbonyl Sampling and Analysis by HPLC

    Sampling of Carbonyls Using a Dual-Layer LpDNPH Glass Sampling Tube and HPLC Analysis with an Ascentis Express C18 Column The dual-layer LpDNPH ORBO-555 tube is an effective sampler for measuring airborne carbonyl concentration. The low background levels in the sampler allow for accurate measurements of low concentration samples or samples collected in ...


    By MilliporeSigma

  • Analysis of Bisphenol A on Ascentis Express RP-Amide

    Analysis of Bisphenol A and Related Compounds on Ascentis™ Express RP-Amide This application report shows the efficient separation of Bisphenol A, Dimethyl Bisphenol A, and Nonylphenol, via HPLC using an Ascentis Express RP-Amide column, under gradient conditions and with UV detection. ...


    By MilliporeSigma

  • USLC columns put the right tools in your LC method development toolbox

    Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection The most significant influence on chromatographic peak separation, or resolution, is column selectivity. Unfortunately, column selectivity is also the least understood and most underutilized parameter. To improve selectivity, method developers often concentrate on ...


    By Restek Corporation

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