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Transcreener - ADP2 Assay Kit - FP Readout
The Transcreener ADP2 Assay with FP readout measures ADP via antibodies selective to ADP and a far-red fluorescent tracer. ADP produced in the reaction competes with the tracer, decreasing fluorescent properties and providing a fluorescent readout. The universal detection method allows screening of virtually any kinase in a high throughput format. The assay produces an excellent signal at less than 10% ATP consumption for a broad range of initial ATP concentrations (0.1-1,000 µM). Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs. Used in over millions of wells by pharma since 2006, Transcreener is the most extensively validated ADP detection method available backed by the best technical support in the industry.
- Direct detection of unlabeled ADP
- Robust Assay Z` > 0.7 under initial velocity conditions
- Far-red fluorescent readouts minimize compound interference
- A safe, non-radioactive method
- Use with ATP concentrations from 0.1 to 1000 µM
- Can be used in continuous or end point mode
- Universal - use with virtually any kinase
- Measure enzymatic Activity of Kinases
- Screen Compound Libraries for Kinase Modulators
- Quantify Inhibitor Potency
- Inhibitor Selectivity Profiling
- Measure Drug-Target Residence Time
Run your enzyme reaction, add Transcreener reagents, and read your plates. The ADP assay is compatible with 96, 384, and 1536-well formats.
Detection of ADP to Measure ADK Enzymatic Activity with FP Readout
Below is an example of the Transcreener ADP2 Assay with FP readout detecting ADP released by Adenosine Kinase. The assay demonstrates linearity when raw data is converted to ADP using a standard curve.
- 384-Well Format
- Z`-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
- Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
- Z` and Δ mP specifications to be met using Transcreener ADP2 Assay Reagents
- Read time to achieve Z` and Δ mP specifications ≤ 5 minutes
Look for these stickers on instruments that have successfully met the validation criteria.
The instrument validation program is a collaborative effort with the major suppliers of multimode readers to insure that Transcreener assays peform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine optimal filters and settings for maximal assay performance. We provide you with application notes with all the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.