Malvern Omnisec Reveal - Advanced Multi-detector GPC/SEC System
Molecular weight , intrinsic viscosity and numerous other parameters can be measured with OMNISEC REVEAL, the world’s most advanced integrated GPC/SEC multi-detector available. It can be added to any existing conventional GPC/SEC system or used in conjunction with OMNISEC RESOLVE to form a complete GPC/SEC solution, to offer comprehensive molecular characterization of synthetic and natural polymers , as well as proteins.
OMNISEC REVEAL can be used to relate a polymer’s molecular properties to its bulk characteristics in a wide range of applications, from the packaging and paint industries to the manufacturing of food additives and pharmaceuticals. Its protein characterization capabilities can be used to study the effects of oligomerization, aggregation and conjugation on activity and efficacy in academic research and biopharmaceutical development.
The ultimate in advanced detection for GPC/SEC
- Complete flexibility in detector configuration. Only purchase the detectors which add value to you now, with the security that any detector you require in the future can be installed onsite
Multi-detection SEC offers unparalleled information on the characterization of polymers, polysaccharides and proteins
- Measuring absolute molecular weight, intrinsic viscosity, hydrodynamic radius, Mark-Houwink parameters, radius of gyration, composition, conformation and more
- Rely on OMNISEC to help you understand, control and optimize your product performance
- Detection and quantification of protein aggregation to drive faster sample characterization and development
Fully integrated temperature-controlled detector module for improved detector stability and sample characterization
- Improve separations and result accuracy with the column oven and temperature-controlled integrated detector compartment – all installed as standard!
- Minimized inter-detector tubing, maintained at the same temperature as the detector, reduces band broadening effects to further increase signal quality
More reliable measurements with market leading light scattering sensitivity
Measure low concentration samples with a highly sensitive and stable refractive index detector
- Measure molecular weight of polymers as low as 200 g/mol!
- Injection masses as low as 100 ng for characterization of samples which were previously unmeasurable using advanced detection
- Make sure you don’t miss low molecular weight and low concentration contaminants or protein fragments
Improved structural comparisons enabled with the unique mechanical auto balance of the differential viscometer
- Boost lab productivity and reduce service costs with a novel self-balancing viscometer design with user exchangeable capillaries
See the full range of your samples UV absorbance with the UV-PDA
- Distinguish chemical changes by measuring absorbance across the full UV/Vis spectrum (190-900nm) using the photodiode array
- All UV channels are recorded giving you the flexibility to choose what wavelength to use in post-processing
All systems can be fully serviced and supported onsite
- No need to worry about shipping systems for service or support, all units can be fully serviced onsite to ensure minimum downtime
Following separation of the sample according to its hydrodynamic volume the sample will pass through 1 or more detectors where its presence is recorded. OMNISEC REVEAL is a fully integrated multi-detection module which can contain up to 4 different detectors.
The RI and UV detectors give information about the concentration of the components in the sample. The light scattering detectors respond to the intensity of light scattered by the sample which is related to molecular weight and also allows Rg of large molecules to be calculated. The viscometer measures the changing solution viscosity to calculate the intrinsic viscosity of the sample which is a measure of structure.
Coupling the efficient sample separation of OMNISEC RESOLVE with the fully optimized, multi-detector, information-rich analysis delivered by OMNISEC REVEAL, Malvern Panalytical has produced a market-leading solution for researchers and formulators developing novel natural and synthetic polymers, pharmaceutical and biopharmaceutical products and foods.
Refractive Index
Measure the concentration of any solute with excellent sensitivity
UV/Vis PDA
Measure absorbance at any wavelength from 190-900 nm
Assess changes in absorbance spectra
Characterize copolymers
Light Scattering
Measure absolute molecular weight in a wider range of applications
Measure as little as 100 ng of material
Measure molecular weights down to 200 Da
Viscometer
Better intrinsic viscosity measurements thanks to automated balancing
Reduced service cost and time thanks to user-exchangeable capillaries
- Dimensions (W, D, H): 420, 640, 600 mm
- Weight: 40 kg
- Detector temperature range: 20 - 65 °C
- Software: OMNISEC software v10 (or later)
- Data acquisition rate: 100 Hz
- Patents:
- US20140060162A1 & EP2619543B1 "Modular capillary bridge viscometer"
- US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"
Detector 1
- Detector: Light scattering
- Measurement principle: RALS 90° angle, LALS 7° angle
- Light source: 50 mW, 640 nm laser
- Cell volume: 18 µL
- Dynamic range: 2500 mV
- Baseline noise: <0.1 mV
- Baseline drift: <0.2 mV/hr
Detector 2
- Detector: Differential refractive index
- Measurement principle: Deflection
- Cell volume: 12 µL
- Dynamic range: ±2.5 x 10-4 RIU
- Baseline noise: <10-7 RIU
- Baseline drift: <3 x 10-7 RIU/hr
Detector 3
- Detector: Viscometer
- Measurement principle: 4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries
- Detector volume: 150 µL/capillary
- Differential pressure dynamic range: ±5000 Pa
- Differential pressure baseline noise: <0.3 Pa
- Inlet pressure dynamic range: 100 kPa
- Inlet pressure baseline noise: 0.01 kPa
- Baseline drift: <0.2 kPa/hr
Detector 4
- Detector: Diode-array-based UV/Vis spectrometer
- Wavelength: 190 - 900 nm
- Number of wavelengths: 1024
- Wavelength accuracy: <1 nm
- Wavelength resolution: 0.6 nm
- Cell volume: 7.5 µL
- Path length: 10 mm
- Baseline noise: 2 x 10-5 AU
- Baseline drift: 5 x 10-4 AU/hr
Measurement type 1
- Measurement type: Absolute molecular weight
- Measurement range: 200 - >107 g/mol
- Minimum quantifiable mass: Using HPLC-SEC:
- 100 ng of 100 kDa molecular weight polystyrene in THF
- 100 ng of BSA in PBS
- Measurement principle: Light scattering (LALS or RALS)
Measurement type 2
- Measurement type: Intrinsic viscosity
- Minimum quantifiable mass: Using HPLC-SEC:
- 0.5 µg of 100 kDa molecular weight polystyrene in THF
- Measurement principle: 4-capillary Wheatstone bridge
Measurement type 3
- Measurement type: Concentration
- Minimum quantifiable mass:
- Using HPLC-SEC:
- 100 ng of 100 kDa molecular weight polystyrene in THF
- 100 ng of BSA in PBS
- Measurement principle: Differential refractive index detection
Operating environment
- Temperature: 15 °C - 30 °C