Alba STED - FLIM/FFS Laser Scanning Nanoscope

The instrument for quantitative cell biology at single-molecule detection. Stimulated Emission Depletion (STED) is a powerful microscopy technique that allows for the observation of fluorescence structure with spatial resolution below the diffraction limit. The Alba-STED uses the pulsed excitation and pulsed depletion approach (pSTED) in combination with the digital frequency domain fluorescence lifetime imaging (FastFLIM) to record the time-resolved photons which allows for an increase in the image resolution and the separation of two labels with the same excitation wavelength.

Most popular related searches

fluorescence imaging

fluorescence spectroscopy

inverted microscope

optical separation

pSTED (Pulsed excitation and pulsed STED)
FastFLIM for time-resolved pSTED acquisition
Improved image resolution using the phasor plot

Dual-label excitation
Fast image acquisition (dwell time: 0.2 µs)
High dynamic range (signal up to 60 million counts/s)

1p or 2p confocal images
FLIM Fluorescence Lifetime Imaging (FastFLIM)
PLIM, Phosphorescence Lifetime Imaging
Polarization images
Fluorescence Fluctuations Spectroscopy (FCS, FCCS, PCS, FLCS)
RICS, N&B, Scanning FCS
Burst Analysis
FRET efficiency determination

Instrument Features	Individual pinholes on each acquisition channel Computer-controlled selection of the pinhole variable aperture Computer-controlled positioning of the pinhole in the imaging plane Single-photon or multi-photon excitation Up to four channel data acquisition Auxiliary port for camera
Acquisition and Analysis Software	VistaVision by ISS
STED laser	Pulsed, 775 nmAverage output power: 1 W Pulsewidth: about 600 ps Repetition rate: 0-100 MHz; or Ext. CLK Beam quality: M² < 1.1, TEM00 Amplitude noise: < 4.0% rms
Excitation laser	Pulsed, 640 nmPulsewidth (at medium power): 40-90 ps Repetition rate: 20, 50, 80 MHz; or Ext. CLK Power (at 50 MHz): up to 5 mW
Laser Launcher	Models for 3-, 4-, 6-laser. Light is delivered to the microscope through a single-mode fiber optic.
FastFLIM	4 separate input signal Dynamic range: up to 15 million counts/s per channel Lifetime range: from picosecond to second
Microscope	Inverted or Upright
Objectives	Air objectives with 20X, 40X, 60X magnification and 1.5-8.1 working distances Oil immersion objectives, 1.4 NA and 60X (standard); other aperture available Water immersion objectives,1.2 NA 60X (standard), with coverslip correction (for 0.15-0.18 coverslip); other apertures available
Stages	Large distance movement (100x100x10 mm) Stepper motor-controlled XYZ stage Micro-distance movements XYZ piezo-controlled stage, 100x100x50 µm with 5 nm step resolution.
Sample Holders	Microwell plates Petri dishes Coverslip
Light Detectors	GaAs PMT (Hamamatsu H7422P models) Hybrid PMTs (Hamamatsu R10467U models) SPADs
Image acquisition	FLIM acquisition: 0.2 µs dwell time
OS Requirements	Windows 10, 64-bit
Power Requirements	Universal power input: 110-240 V, 50/60 Hz, 400 VAC
Dimensions	885 mm (L) x 600 mm (W) x 330 mm (H)
Weight	40 kg

Alba STED - FLIM/FFS Laser Scanning Nanoscope

Key Features of Alba-STED for FLIM/FFS:

Measurements with Alba-STED for FLIM/FFS:

Specifications for Alba-STED for FLIM/FFS