QuantiFluo - Model DNH3-200 -Ammonia Assay Kit

Key Features

• Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL360/450nm

Samples

• Biological (eg urine) and environ-mental samples

Species

• All

Size

• 200 tests

Detection Limit

• 12 μM

Shelf Life

• 6 months

More Details

• AMMONIA (NH₃) or its ion form ammonium (NH₄⁺) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH₃ are very desirable. BioAssay Systems ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 360/450nm) is proportional to the ammonia concentration in the sample.