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BioLegend, Inc.
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  5. Alexa Fluor - Model 488 - Anti-Mouse ...

Alexa Fluor - Model 488 -Anti-Mouse IgD Antibody

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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.

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Isotype Control:Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl
Reactivity:Mouse
Antibody Type:Monoclonal
Host Species:Rat
Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration:0.5 mg/ml
Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Application Notes:The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3.
Application References:
(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
Product Citations:
  1. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  2. Katsuyama T, et al. 2019. J Clin Invest. 129:5411. PubMed
  3. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  4. Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed
  5. Ly A, et al. 2020. Cell Reports. 29(8):2257-2269.e6.. PubMed
  6. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
RRID
AB_10730618 (BioLegend Cat. No. 405717)
AB_10730619 (BioLegend Cat. No. 405718)
Structure:Ig family
Distribution:B cells
Function:B cell differentiation
Cell Type:B cells
Biology Area:Immunology
Gene ID:380797 View all products for this Gene ID
UniProt:View information about IgD on UniProt.org