BioLegend, Inc. products

Conventional/traditional” flow cytometer use a series of mirrors and filters in order to capture the emission wavelengths from excited fluorophores. Fluors that have emission profiles that fall into the same filter cannot be separated or identified from one another. Our spectra analyzer can display the excitation and emission spectra for dozens of fluorophores. This allows you to compare the excitation and emission profiles of fluors, custom filters, and laser lines to see what best fits your cytometer.

Spectral cytometers capture the full spectrophotometric profile of the fluorophores across all lasers. The fluors’ profiles are captured in 10-30 nm segments across the emission range to accurately unmix the fluorophores. This allows fluors that normally cannot be unmixed on a conventional cytometer (e.g. Pacific Blue™ and Brilliant Violet 421™) to be used together on an instrument like Cytek’s Aurora. As such, this web tool is dedicated to displaying the emission spectra of fluorophores based on Cytek’s Aurora spectral cytometers.

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10^-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10^-14 mol/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.