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Alexa Fluor - Model 700 -Anti-Mouse IgD Antibody
FromBioLegend, Inc.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
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- Isotype Control:Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl
- Reactivity:Mouse
- Antibody Type:Monoclonal
- Host Species:Rat
- Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation:The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- Concentration:0.5 mg/ml
- Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application:FC - Quality tested
- Recommended Usage:
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer`s capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser:Red Laser (633 nm)
- Application Notes:The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3.
- Application References:
(PubMed link indicates BioLegend citation)
- Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
- Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
- Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
- Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
- Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
- Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
- Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
- Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
- Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
- Product Citations
- Carbonetti S et al. 2017. Journal of immunological methods. 448:66-73 . PubMed
- Staffas A et al. 2018. Cell host & microbe. 23(4):447-457 . PubMed
- Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
- Yen WF et al. 2019. Cell reports. 27(5):1472-1486 . PubMed
- Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
- RRID
- AB_2563340 (BioLegend Cat. No. 405729)
AB_2563341 (BioLegend Cat. No. 405730)
- Structure:Ig family
- Distribution:B cells
- Function:B cell differentiation
- Cell Type:B cells
- Biology Area:Immunology
- Gene ID:380797 View all products for this Gene ID
- UniProt:View information about IgD on UniProt.org