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BioLegend, Inc.
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  5. APC - Anti-Mouse IgD Antibody

APC - Anti-Mouse IgD Antibody

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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

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Isotype Control:APC Rat IgG2a, κ Isotype Ctrl
Reactivity:Mouse
Antibody Type:Monoclonal
Host Species:Rat
Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3.
Application References:
(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
Product Citations
  1. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  2. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  3. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  4. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  5. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  6. Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
  7. Kitabatake M, et al. 2012. J Immunol. 189:1193. PubMed
  8. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  9. Tian M, et al. 2020. Proc Natl Acad Sci U S A. 117:7929. PubMed
  10. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  11. Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed
RRID
AB_10645480 (BioLegend Cat. No. 405713)
AB_10643423 (BioLegend Cat. No. 405714)

Structure: Ig family
Distribution: B cells
Function: B cell differentiation
Cell Type: B cells
Biology Area: Immunology
Gene ID: 380797 View all products for this Gene ID
UniProt: View information about IgD on UniProt.org