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ASI - Model CRISP -Autofocus System for Microscopy
The CRISP Autofocus System is intricately designed to maintain precise focus during microscopy operations. It compensates for thermal variations and other factors that can lead to sample drift during prolonged observations. When integrated with the Dual C-Mount Splitter (DCMS), it provides a robust focus stabilization solution that supports both analog and high-resolution digital camera inputs. CRISP detects focus shifts by projecting an IR LED illuminated mask, capturing the focus error through lateral motion detection. This enables closed-loop control for focus adjustment. It is typically mounted with ASI's Dual C-Mount Splitter, housing the necessary dichroic beam combiner and blocking filters. Note that CRISP relies on reflected light from sample interfaces with varying refractive indices and is not suitable for index matching mounts. Its specifications delve into adjustments and options, enhancing its versatility for sophisticated microscopy applications.
The CRISP system is designed to maintain focus over time, i.e. compensate for thermal and other factors that may cause the sample to drift. It also can be used to maintain a given focal point while scanning the sample in XY. If you are looking to find the optimal focal point while scanning through the sample in Z please see our video autofocus system.
CRISP is parted with the Dual C-Mount Splitter (DCMS), camera and tube lenses as part of a microscope. The Dual C-Mount Splitter (DCMS) provides two parfocal C-Mount ports when mounted on many common microscopes. Typical combines a fast analog autofocus camera system simultaneously with a high-resolution digital camera.
- Mounts onto any microscope’s standard C-Mount port
- Maintains ideal focus for days
- Works with most normal microscope objectives
- Low noise electronics allows locking to glass/water interfaces
- Integrates with ASI Piezo-Z or motorized focus stages
- Simple post-lock fine adjustment of focus
- Automated control
- Built-in C-Mount extension for optical offsets
- LED beam iris to match illumination beam to objective pupil for optimum performance
- Lateral detector adjustment
- LED colors from 625-1050 nm
- LED intensity control
- Programmable gain and averaging functions to optimize system for stability or speed
- Light Source : LED
- Wavelength : Choose from 625-1050 nm
- Optical Interface : C-Mount
- Typical Focus Accuracy : <5% of objective Depth of Focus
- Controller : MS-2000 w/ CRISP card
- TG-1000 w/ CRISP card