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ChemoMetecModel Via1 -Cassette

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The Via1-Cassette is a specialized cell sampling and staining device for viability and cell count applications with NucleoCounter® instruments. It contains the fluorophores acridine orange (AO) and 4’,6-diamidino-2-phenylindole (DAPI), which are immobilized within the cassette’s channels.

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  • Product number: 941-0012
  • Size: 100 pcs.
  • Storage: Room temperature

Approximately 60 µl of a cell sample is loaded into the cassette, dissolving AO and DAPI dyes in the mixing channels. AO stains both live and dead cells, providing a total count, whereas DAPI stains the dead cells.

Each cassette carries an individual dot-code indicating the exact volume of its counting chamber, which is read by the NucleoCounter® to calculate the exact cell concentration. The total volume of analysis within the counting chamber is approximately 1.4 µl.

Although trypan blue is a commonly used cell staining dye, it is a carcinogen. Therefore, operators are potentially exposed to the dye when pipetting and staining cell samples. The Via1-Cassette™ is a safe, cell counting system that does not contain trypan blue. In addition, any cell handling prior to counting occurs inside the cassette, thereby shielding the user from potential hazardous cell material or toxic staining reagents. For more information on safety, please see the Via1-Cassette™ Conformity Statement.

The Via1-Cassette™ is used with the NucleoCounter® NC-200™ and NC-3000™.

  • Used with NucleoCounter® NC-200™, NucleoCounter® NC-3000™
  • Dye Acridine orange (AO) and DAPI
  • Loading volume 60 µl
  • Analysis volume 1.4 µl
  • Samples/cassettes 1