OLS-Omni - Model CASY -Cell Counter and Analyzer

CASY measures the conductivity between 2 electrodes separated by a defined pore. Cells are suspended in CASYton, a defined buffer solution. During measurement, cells passing through the pore generate an electrical pulse. The intensity of the signal directly correlates with the volume of the cell. Hence CASY detects the volume of each cell passing through the pore.

For hundreds of our GMP/GLP customers we regularly recertify a consistent precision allowing a maximum variation below ±2%.

See life as it is – stain free viability control.

CASY’s superior detection technology combining Electronic Current Exclusion (ECE) and Pulse Field Analysis (integration of cell signal at 1MHZ while passing through the pore) enable the accurate determination of viability without any influence by staining, focusing, etc.

Viable cellsare detected with full cell volume as the cell membrane provides a barrier for current.
Dead cells have lost resistance from cytoplasmic membrane. Instead the much smaller volume of the nucleus is detected that can very well be distinguished.

Aggregation – nothing to worry about.

As CASY determines the volume of each cell with a precision of ±2%, it does the same with aggregates. Unlike optical measurements which either ignore aggregates or try to draw some rings into rather low contrast images of them, CASY uses mathematically correctvolume-based aggregationcorrection. Basically, CASY will see how often a single cell fits into each aggregate detected. Mean Volume and Mean Size even indicate changes in the level of aggregation, for example when working with induced Pluripotent Stem cells (iPSC).

The only thing CASY cannot beat is statistic– CASY provides precise, accurate and reproducible results over a wide range of cell concentration. The only limit is given by statistical significance. In other words, counting only 300 cells will result in higher variation and standard deviation compared to counting more than 1.000 or even 10.000 cells per measurement. But even for very low concentrated cells, especially when they are very valuable to you or limited in amount, CASY has a solution to improve results – simply increase the measured volume to up to 3.6ml.

Here customers from Switzerland prepared 6 measurements of keratinocytes and compared the results. Even before looking at the results in numbers, the overlay of size distributions shows thehigh reproducibility of results. The high reproducibility which is independent of users and locations make CASY a great instrument in Biotech or Pharma especially when operations are mirrored at different locations.