Sigma-Aldrich

Cellulose HPTLC Plates

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Cellulose is an organic sorbent, which is particularly suitable for the separation of hydrophilic substances by partition chromatography. EMD Millipore’s high-quality cellulose plates include classical TLC or HPTLC grades, and facilitate a wide range of separations. Both plates are available with or without a special fluorescent indicator, which is stimulated to intense blue fluorescent emission at long-wave UV light of 366 nm, and at short-wave UV light of 254 nm.

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TLC cellulose layers: Based on microcrystalline cellulose for standard separations

HPTLC cellulose layers: Based on high-purity, rod-shaped microcrystalline cellulose, resulting in a highly reduced diffusion of analytes for critical high-performance separations

  • Ideal for partition chromatography of hydrophilic substances
  • High-performance separations due to greatly reduced diffusion of analytes
  • Easier detection through intense fluorescent indicator

Typical applications of cellulose plates include the analysis of amino acids, carbohydrates, phosphates, nucleic acids and nucleic acid derivatives for:

  • Two-dimensional separations, such as amino acid “fingerprints”
  • Metabolic studies