Sherwood - Model 360 -Clinical Flame Photometer

The Model 360 has a Calcium filter as standard. It must be noted however that in a propane/air flame there is insufficient energy to break calcium phosphate bonds which may occur within samples. In these samples a pre-treatment protocol must be used. We have a method that can be viewed here: Method F-003M Calcium in Biological Fluids. With other Calcium samples chelation with EDTA or extraction with Mehling`s 3 solution may be indicated. You should also be aware of interference with Calcium determinations if Sodium is present in your samples.

The detector response in the Clinical 360 is "linearized" when the Sodium filter is selected; as is the expectation for a Clinical Flame Photometer. The specification assumes samples are diluted with de-ionized water as indicated.

Measurement Range

• Na Serum: 110 to 170 mmol/l
• Na Urine: 0 to 200 mmol/l
• K Serum: 0 to 10.0 mmol/l
• K Urine: 0 to 200 mmol/l
• Li Serum: 0 to 3.0 mmol/l

Reproducibility

• Na (140 mmol/l) better than 1.5% CV
• K (5 mmol/l) better than 1.5% CV
• K (80 mmol/l) better than 1.5% CV
• Li (1.5 mmol/l) better than 2.5% CV

Linearity

• Na Serum within ± 2 mmol/l
• Na Urine within ± 4 mmol/l
• K Serum ‹ ± 0.2 mmol/l
• K Urine ‹ ± 2 mmol/l
• Li Serum ‹ ± 0.2 mmol/l

Dilution Factor

• Na Serum/Urine: 1 in 200 with de-ionised water
• K Serum: 1 in 200 with de-ionised water
• K Urine: 1 in 1000 with de-ionised water
• Li Serum: 1 in 50 with de-ionised water

Note: The above is correct for use with Propane or Butane. Results obtained from 20 replicates of the same sample, aspirating sample for 20 seconds, then water for 10 seconds. To achieve the stated specification the flame must be alight for a minimum of 30 minutes, with diluent being aspirated. (For a brand new, newly installed unit, that stabilisation is highly likely to be greater than 30 minutes).

Note: For Li determinations; blank and standard solutions must contain similar levels of Na, K and Ca to those present in physiological samples.

• 3-6 ml/min

• Time to stability 20 seconds

• Analogue: Voltage 0.20 Volts FSD

(h x w x d)
500 mm x 300 mm x 230 mm 5.6 Kg Nett
480 mm x 420 mm x 350 mm 8.6 Kg Packed