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Sepax Unix - Model SEC -300 -Columns
Utilizing proprietary surface technologies, Unix SEC-300 phase is made of uniform, hydrophilic, and neutral nanometer thick films chemically bonded to high purity and mechanically stabilized silica with the particle size of 1.8 µm. The combination of small particle size and large pore volume of Unix SEC-300 renders the highest separation efficiency and resolution of analytes. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility. Our unique bonding chemistry, coupled with the maximized bonding density, allows Unix SEC-300 to provide high stability and negligible non-specific interactions. Typical applications for Unix SEC-300 columns include separation and analysis of biological molecules and water-soluble polymers.
- Phase: UnixTM SEC-300
- Material: Neutral, hydrophilic film bonded silica
- Average Particle size: 1.8 µm
- Pore size: 300 Å
- Protein MW range (native): 5,000 - 1,250,000 Da
- pH stability: 2.0 – 7.6 (up to pH 8.5 can be tolerated temporarily*)
- Backpressure for 4.6×150 mm(0.35 mL/min): ~1700 psi
- Maximum backpressure: ~4500 psi
- Maximum salt: Up to 500 mM
- Maximum temperature (°C): ~4 – 80°C
- Mobile phase compatibility: Aqueous and organic
- Particle size of 1.8 µm with 300 Å pore
- Ultra high separation efficiency, capacity, and resolution
- High stability over low and high concentration salt
- Lot-to-lot reproducibility
- Excellent biomolecule separation while maintaining its original conformation due to low pressure resin property (up to 50% lower than competitor)
- >50% faster separation than traditional SEC columns without compromising resolution for high throughput screening
- Ideal for separation and analysis of biological molecules such as mAb, ADC, proteins, protein conjugates, and oligonucleotides
- Ideal for separation and analysis of natural and synthetic polymers, and nanomaterials