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- Sepax Proteomix - Model HIC - Columns
Sepax Proteomix - Model HIC -Columns
ProteomixHIC columns are specially designed for high resolution and high efficiency separations of proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), oligonucleotides and peptides via a hydrophobic interaction chromatography (HIC) mechanism. Utilizing proprietary surface technologies, Proteomix® HIC-NP resin is made of non-porous polystyrenedivinylbenzene (PS/DVB) beads with narrow-dispersed particle size distribution. As shown in figure below, the PS/DVB bead is modified with alkyl groups or aryl groups that provide hydrophobic interaction with analytes.
Proteomix® HIC-NP resin is highly rigid and mechanically stable. In comparison to silica-based HIC phase media, Proteomix® HIC-NP phases have advantages for biomolecule separations with a wide pH range (2-12) and high chemical stability. The nonporous structure, unique chemistry, and narrow particle distribution offer special selectivity, high-resolution separation of proteins such as mAb, ADC and related protein fragments, DNA and oligonucleotides. Proteomix® HIC-NP media is applicable at laboratory discovery, laboratory-scale purification and preparative chromatography for the production of several milligrams to grams of proteins. Sepax also provides polymethacrylate-based Generik MC and Polar MC HIC 30 µm media which offers similar characteristics to the Proteomix® HIC-NP but is designed for process-scale applications from grams to kilograms of proteins.
- Resin Matrix: Spherical, highly cross-linked PS/DVB
- Pore Size: Nonporous
- Particle Size: 1.7, 5 and 10 µm
- Phase Structure: Ethyl, Propyl, Butyl, and Phenyl (5, 10 µm)
- Ethyl and Butyl (1.7 µm)
- Separation Mechanism: Hydrophobic interaction
- pH Stability: 2-12
- Operating Temperature: Up to 80 °C
- Operating Pressure: 6000 psi (5, 10 µm)
- 8000 psi (1.7 µm)
- Mobile Phase Compatibility: Compatible with aqueous solution, a mixture of water and acetonitrile, acetone, methanol, or THF
- Highest capacity and resolution
- High protein recovery with intact biological activity
- High stability and lot-to-lot reproducibility
- High pressure and high temperature tolerance
- Ideal for separation and analysis of hydrophobic proteins, monoclonal antibodies and their derivatives such as antibody drug conjugates, and organic molecules derivatized with polymer branches