ONI - Complete Package Nanoimager for Super-Resolution Microscopy
20nm Resolution: Our combination of imaging modalities and advanced software allows single molecules to be observed and tracked with high sensitivity. Autofocus in Realtime: Rapid and precise stabilization of the microscope focus, with locking technology to minimize Z-drift. Correction: Post acquisition drift correction with nanometer precision Temperature control: Precise control and measurement of temperature, with heating elements to keep the entire microscope unit at a stable, uniform temperature. Ideal for live cell imaging, facilitating single-particle tracking. Powerful lasers: Single molecule localization microscopy in multiple colors and 2 channels. Up to 1W laser power at the source. 8-12 kW/cm² power density at the sample plane. Micro fluidics compatible: Powerful solutions to control sample conditions. DNA paint and RNA hybridization. Up to 16 channels. Automation of sample preparation.
- 4 Lasers
- 1.6 RMS read noise
- 2 Channels
- 3D Included
Imaging technology
- 2D and 3D single-molecule localization microscopy (SMLM)
- 3D single-particle tracking (SPT)
- Total internal reflection fluorescence (TIRF)
- Förster resonance energy transfer (FRET)
Spatiotemporal resolution
- Up to 20nm in XY, 50nm in Z via SMLM
- Up to 10ms / 100Hz with SPT, FRET, TIRF
Imaging channels
- 4 interlaced, 2 simultaneous
Field of view
- 50μm x 80μm with a 100x objective
- 250μm x 400μm with a 20x objective
Illumination
- 405nm, 488nm, 561nm, 640nm with up to 1W
- Up tp 10 kW/cm² in the sample plane (integrated power sensors)
- Flat-field homogeneous laser illumination
- Continuous illumination angle from epi, to HILO to TIRF
Light patterning
- Digital micromirror device (DMD) based light patterning
Objectives
- Numerical aperture (NA) fom 0.45 to 1.50
- Magnification from 20x to 100x
Dimensions
- Nanoimager: 21.5cm (w) x 21.5cm (d) x 15.5cm (h)
- Light Engine: 21.5cm (w) x 42.0cm (d) x 45.0cm (h)