Sage Science specializes in advanced DNA and RNA sequencing systems designed for a wide range of applications in genomics. The PippinHT, BluePippin, and Pippin Prep systems provide highly precise DNA size selection, with capabilities ranging from 100 bp to 1.5 kb and beyond, supporting up to 24 samples per run. These systems offer high-pass filtering up to 40 kb, enhancing their utility for various sequencing needs. Additionally, the HLS2 HMW Library System enables Cas9-assisted target selection and HMW DNA extraction, facilitating large genomic region analysis. SageELF enables whole sample fractionation for DNA and proteins, while the TELL-Seq Kit allows barcode-linked library preparation for NGS. The SageHLS system integrates components for running electrophoresis at 25V to 100V pulsed field, supporting targeted DNA purification via CRISPR/Cas9. These systems are engineered to support next-generation sequencing with high precision and are suitable for both short-read and long-read platforms, making them versatile tools in modern genomic research.
CRISPR/Cas9 can be used to design targeted purification of large genomic regions or genes. This in vitro application with Cas9, termed CATCH, can enhance study of structural variation and provide valuable phasing information.
*Cas9-Assisted Targeting of CHromosome Segments
Jiang et. al., 2015, Nature Communications, doi:10.1038/ncomm9101
SageHLS vs HLS2: The HLS2 offers the same capabilities as the original SageHLS except for the capability to heat or cool the agarose gel cassettes. This has been deemed unnecessary for HLS-CATCH and supported applications.
Sage Science will continue to sell and support the SageHLS platform for researchers who wish to explore active temperature functionality with HLS-CATCH or other studies.
Instrument
SageHLS Specifications :
