Sage Science, Inc. products

The Pippin Prep DNA Size Selection System is a versatile tool designed to optimize library construction for next-generation sequencing platforms, notably endorsed by Illumina and Ion Torrent for specific workflows. This system simplifies the selection of DNA fragments ranging from 100 bp to 1.5 kb, utilizing pre-cast agarose gel cassettes available in various concentrations. This feature ensures accurate targeting for short read sequencing requirements. Capable of processing up to five samples per gel cassette, the system operates efficiently with run times as brief as 30 minutes, ensuring no cross-contamination. The instrument includes built-in optical detection and a CPU, eliminating the need for an external computer. With constant electrophoresis voltage options of 100V or 150V, it supports a stable and reproducible electrophoresis process. Essential standards and reagents accompany the disposable gel cassettes, offering a comprehensive package for effective workflow implementation in DNA size selection.

The BluePippin DNA Size Selection System by Sage Science is designed to provide precise selection and collection of DNA fragments ranging from 100 base pairs to 50 kilobases. Utilizing pulsed-field electrophoresis, it facilitates the separation of high molecular weight DNA, crucial for long-range genomic studies. Users can set specific size thresholds via software, allowing high-pass filtering to collect fragments above the desired size. This system enhances process efficiency by accommodating up to five samples per gel cassette, minimizing cross-contamination risks. Available in different agarose concentrations, the cassettes offer flexible size selection. The BluePippin employs constant voltage settings with options for pulsed fields to cater to a variety of applications. Integrated with a built-in CPU and optical detection capabilities, it simplifies operations by eliminating the need for external computing devices. The system supports faster run times, high input capacity, and ensures reliable DNA size selection for advanced research applications.

The PippinHT DNA Size Selection System by Sage Science offers an advanced solution for high-throughput next-generation sequencing laboratories. This system accommodates up to 24 samples per run, providing significant efficiency gains over predecessors such as the Pippin Prep and BluePippin, which handled fewer samples. It supports the selection of DNA fragments ranging from 100 bp to 1.5 kb for size selection and up to 40 kb for high-pass protocols. The automated system features a built-in CPU, eliminating the need for an external PC, and includes components like a monitor, keyboard, and mouse. Operating at a constant 75V for electrophoresis, the system includes pre-cast gel cassettes and all necessary standards and reagents. With precise optical detection capabilities (470 nm excitation and 525 nm emission), the PippinHT is engineered for busy sequencing labs that require reliable and scalable DNA size selection solutions.

Sage Science specializes in advanced DNA and RNA sequencing systems designed for a wide range of applications in genomics. The PippinHT, BluePippin, and Pippin Prep systems provide highly precise DNA size selection, with capabilities ranging from 100 bp to 1.5 kb and beyond, supporting up to 24 samples per run. These systems offer high-pass filtering up to 40 kb, enhancing their utility for various sequencing needs. Additionally, the HLS2 HMW Library System enables Cas9-assisted target selection and HMW DNA extraction, facilitating large genomic region analysis. SageELF enables whole sample fractionation for DNA and proteins, while the TELL-Seq Kit allows barcode-linked library preparation for NGS. The SageHLS system integrates components for running electrophoresis at 25V to 100V pulsed field, supporting targeted DNA purification via CRISPR/Cas9. These systems are engineered to support next-generation sequencing with high precision and are suitable for both short-read and long-read platforms, making them versatile tools in modern genomic research.

The Pippin Pulse Electrophoresis Power Supply is an advanced tool designed to enhance DNA analysis in research settings. By separating the pulsed-field power supply from the BluePippin instrument, Sage Science offers an affordable solution for resolving DNA to 100 kb and beyond. This system is particularly suitable for researchers looking to develop custom field-inversion protocols, thanks to its user-friendly software interface. The power supply operates within a voltage range of 25-175V and supports currents up to 200 mA, making it versatile for various electrophoresis applications. The package includes the power supply unit, connecting cables, a software program for PC-based configuration, and a comprehensive user manual. The Pippin Pulse is engineered to meet the demands of next-generation genome mapping, providing precise and efficient performance for scientific laboratory environments.