TERMIPol - DNA Polymerase

• MALDI-TOF mass spectrometry
• Primer extension
• Mass-array

• Concentration: 5 U/µl
• Hot-start: no
• Error rate per nucleotide per cycle is app. 8 x 10-5
• Estimated half life at 95ºC is 1.5 hours.
• Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
• Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
• Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC.

Thermostable DNA polymerase for highly efficient incorporation of unconventional nucleotides.

• efficient incorporation of ddNTPs, acyNTPs, or fluorescent nucleotides
• the assay success rate of 99% in MALDI-TOF
• robust and reliable
• reaction set-up and shipment without dry ice