Solis BioDyne products

FIREPol is a highly processive, thermostable DNA Polymerase. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month. Recommended for routine applications (fragment up to 3 kb from genomic DNA). Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning. The fidelity of FIREPol is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10-5).

HOT FIREPol GC Master Mix has been developed for working with difficult GC-rich templates and DNA secondary structures. It shows excellent amplification with templates up to 79% GC content. The master mix contains hot-start Taq polymerase HOT FIREPol®, MgCl2, dNTPs, and a special buffer for high yield GC-rich amplification. Extra vials of 100% DMSO and 25 mM MgCl2 enable flexibility in reaction optimization.

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water. We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.

HOT FIREPol® Probe Universal qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes. Proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets. HOT FIREPol® Probe Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the 5` flap endonuclease activity. The Mix contains internal passive reference dye that is compatible with most qPCR cyclers including high ROX or low ROX reference signal requiring platforms. The Mix contains dUTPs to prevent cross-contamination with UNG treatment.

Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye. It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

SOLIScript® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.

RiboGrip™ RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A. RiboGrip™ inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. This novel inhibitor  has enhanced stability at room temperature with no loss of activity for up to 1 month.

FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT-qPCR (quantitative reverse transcription PCR).

TERMIPol DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’→ 3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs, acyNTPs, fluorescent dNTPs and labeled ddNTPs).