Omega Bio-tek, Inc.
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- E.Z.N.A. - DNA/RNA Isolation Kit
E.Z.N.A. - DNA/RNA Isolation Kit
The E.Z.N.A.® DNA/RNA Isolation Kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenized in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind® RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, the maximum yield of DNA and RNA can be purified from the entire sample. Simultaneously isolate DNA and RNA from same cells or tissues using spin column.
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FEATURES: SPECIFICATIONS
- Downstream Application: PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
- Elution Volume: 40-70 µL for RNA; 50-100 µL for DNA
- Starting Material: Cultured cells and animal tissues
- Starting Amount: Up to 1X107 cells; up to 30 mg tissue
- RNA Yield: RNA in one fraction; DNA in another
- Processing Mode: Manual, centrifugation
- Throughput: 1 - 24
- RNA Binding Technology: Silica Mini spin column
- Binding Capacity: 100 µg for DNA and RNA
