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DNAble - Molecular Detection of CMM in Tomato
Our patented DNAble chemistry offers user-friendly isothermal DNA amplification assays that are: Quick, Sensitive, Accurate. The first commercialized on-site GMO DNA detection method, DNAble is a simple, rapid nucleic acid amplification method similar to PCR. However, DNAble amplifies targets in a fraction of the time, with minimal equipment requirements in a non-laboratory setting. Unlike PCR, DNAble shows little inhibitory effects from crude extraction samples. As a result, DNAble allows for highly specific detection, with sensitivity similar to PCR results but with minimal sample preparation.
DNAble chemistry uses a nicking enzyme and a strand displacing polymerase. These enzymes work together at one temperature to achieve exponential DNA amplification and can be used for end-point or real-time quantitative results. Sequence-specific molecular beacon(s) are then employed to detect the amplified target and provide a second level of specificity.
- Simple Protocol
Basic steps with simple transfers
No expertise needed - Crude sample prep
No specialized equipment needed
Fast sample processing - One reaction step
Reverse transcription and amplification take place in same tube - Rapid time to result
< 10 minute amplification
Time to results is less than 20 minutes - Point-of-need capability
Tests can be performed on a battery operated instrument in any setting
Decisions can be made on-site, in real time