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Model iST-NHS -Fast, Reliable, Reproducible Sample Preparation for Chemical Labeling

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Fast, reliable, reproducible sample preparation for chemical labeling (iTRAQ and TMT). The challenge: Chemical labeling reagents have specific handling and compatibility requirements to achieve good labeling efficiencies. Combined with the multi-step methods of proteomics sample preparation for mass spectrometry analysis, chemical labelling protocols have inherent challenges of time, contamination and sample loss.  This challenge is exacerbated with large numbers of samples and small starting amount of input material. The solution: PreOmics® iST-NHS sample preparation is fully compatible with chemical labeling reagents. Allowing streamlined peptide processing, chemical labeling and an efficient peptide clean-up step. 
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1. Samples

Samples should be liquid, homogenates, or soft pellets.

2. Lyse, reduce, alkylate

Add LYSE reagent, incubate for 10 mins at 95°C.

3. Digest and label

Cool, add DIGEST reagent, incubate for 60 mins at 37°C. Label according to vendor instructions.

4. Purify

Load onto the cartridge, WASH and ELUTE. Dry and resuspend in LOAD.

5. MS analysis

Inject and analyze to see improved peptide identities.

While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics® kits take the same time as flying from our Munich HQ to London or from Boston to Chicago. Increase your sample throughput, improve quality and produce reliable data. It’s as easy as i-S-T!

Smile
Improved sample quality
  • High reproducibility: R2>0.9
  • Working range: 1-100 µg
  • Increased peptide and protein identifications
  • Shipped at RT
Relax
Quicker methodology
  • 2.5 hours total time, including labeling
  • Single step incorporating lysis, reduction and alkylation
  • All-in-one solution, all reagents provided
  • Compatible with standard lab equipment

Realize

Cleaner peptides ready for analysis
  • Dual clean up step removes hydrophilic and hydrophobic contaminants
  • Achieve ready to measure peptides
  • Reduce mass spectrometry downtime
  • Manual to fully automated processing options available