Femtonics FocuSpinne - Femto 3D Atlas and FEMTOSmart Modules for Enhanced Microscopy
Enhance your in vivo measurements effortlessly with the Femtonics FocusPinner, which offers Real-Time Motion Correction along X,Y and Z (axial) direction, effectively eliminating motion artifacts. Neuroscience relies more heavily on in vivo measurements of neuronal activity to study cognition, memory, CNS diseases, behaviour, neurochemistry, and many other aspects of the field. One of the bottlenecks of in vivo functional measurements is motion artifacts caused by the movement of the animal, respiration, circulation, or any of the numerous other sources. Here we offer a real-time solution for in vivo motion correction which eliminates artifacts caused by heartbeat, breathing or physical activity at over 100 kHz speed allowing calcium and voltage imaging from spines, dendrites, axons and somata with high resolution.
- Acousto-optic based: By leveraging the capabilities of 3D random-access line scanning, our system enables seamless motion correction along each axis. It offers a significant advantage over the currently available XY plane cross-correlation-based solutions by providing feasible Z directional (axial) correction.
- Useful at in-vivo measurements: motion artifacts caused by the movement of the animal, respiration, circulation can be efficiently eliminated (average residual motion < 10%).
- Real-time: During the measurement, the focal point is on-line relocated based on the estimated motion of the reference point. Compared to quasi-real time solutions the reaction time is one-tenth.
- Reaction time: The cycle of repeated motion correction is less than 100 µs, meaning that the largest residual motion amplitude is the one collected during this short time-window.
- Scanning modes: It is compatible with all scanning modes (e.g. raster, chessboard, multicube, longitudinal and transverse ribbon, snake, Z-Stack) of the FEMTO3D Atlas system.