Solis BioDyne
  1. Companies
  2. Solis BioDyne
  3. Products
  4. FIREPol - Optimized Ready-To-Use PCR ...

FIREPolOptimized Ready-To-Use PCR Master Mix

SHARE

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water. We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.

Most popular related searches
  • suitable for templates up to 5 kb
  • reaction set-up and shipment without dry ice
  • all-in-one master mix format reduces pipetting errors and saves time
  • amplicons are suitable for Sanger sequencing post-PCR
  • Concentration: 5x
  • Hot-start: no
  • Ready to load: no
  • Fidelity: same as Taq
  • Cloning Type: T/A Cloning
  • Amplicon Size: up to 5 kb
  • FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
  • 5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
  • 12.5 mM MgCl21x PCR solution – 2.5 mM MgCl2
  • 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP