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FIREPol - Optimized Ready-To-Use PCR Master Mix
FromSolis BioDyne
Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water. We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.
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- suitable for templates up to 5 kb
- reaction set-up and shipment without dry ice
- all-in-one master mix format reduces pipetting errors and saves time
- amplicons are suitable for Sanger sequencing post-PCR
- Concentration: 5x
- Hot-start: no
- Ready to load: no
- Fidelity: same as Taq
- Cloning Type: T/A Cloning
- Amplicon Size: up to 5 kb
- FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
- 5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
- 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
- 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
