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Fluidity One-M - Protein Interaction Analyzer
Fluidity One-M is an advanced protein interaction analyzer designed for expeditious and accurate molecular interaction studies. The device simplifies the analysis process with a straightforward mix-and-measure workflow that requires no training, allowing users to generate data within 25 minutes. It measures 24 data points per assay, providing comprehensive binding curves and KD values without the need for protein immobilization or purification. By directly measuring in solution, it retains proteins in their native state, ensuring genuine interaction assessments. The analyzer is versatile, accommodating assays at physiological temperatures of 37 °C, thus mimicking natural conditions. Fluidity One-M also quantifies molecular sizes, binding affinities, and stoichiometry. It counteracts traditional methods' limitations through its robust design, favoring cell lysates alongside serum and plasma analyses. The analytics platform, Fluidity Insight, utilizes machine learning to refine experiment designs, enhancing data quality. The analyzer's low maintenance and avoidance of cross-contamination facilitate seamless operation.
Measuring molecular interactions can be tough. But we’ve got your back.
Start simple in less than 1 day
Your time is precious, it should be spent on exploring the insights, not getting lost among complex protocols. Get started easily and confidently with a simple mix-and-measure workflow. No training is required.
Get data delivered in 25 minutes
Fluidity One-M is built to help you move faster to insights. It takes 25 minutes to generate 24 data points, providing a full binding curve and KD value. Go grab some lunch and come back to see your publication-ready results.
Measure interactions as they really are
Direct in-solution measurement – without immobilization or purification – reveals true protein behaviors in their native state. Assays can be run at 37 °C, allowing measurements under conditions that closely mimic physiology.
See a full picture without assumptions
Affinity alone can’t tell a complete story about how proteins truly interact. Fluidity One-M provides multiple metrics to help you fully assess the intricacies of protein interactions.
Molecular size
Fluidity One-M measures molecular size (hydrodynamic radius) of a sample by evaluating its diffusion in a microfluidic chamber.
Binding affinity
The size of the protein is measured in free form and against a titration of binding partner, allowing construction of a binding curve and calculation of binding affinity (KD).
Calibration-free concentration
The concentration of active binding sites can be determined by measuring binding at different concentrations of the labelled protein.
Stoichiometry
Concentration data can also be used to determine binding stoichiometry (number of binding sites per molecule).
Measure in solution, break the constraints
Swiftly overcome the common challenges of traditional protein characterization methods and explore the behaviours of any proteins in their native environments.
No immobilization
With Fluidity One-M, you can measure interactions directly in solution without having to immobilize your proteins on a surface.
No purification
No purification is needed. Fluidity One-M can work with crude samples, saving you preparation time and simplifying the workflow.
No background constraints
Confidently tackle complex backgrounds such as serum, plasma or cell lysate that are difficult for traditional methods.
No target constraints
Analyze any targets from traditional to challenging ones, such as protein aggregates, membrane proteins, disordered proteins, and PROTACs.