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TELOS MicroPlate - Model SPE -For Small Volume Biological Fluids
Developments in LC-MS/MS detection levels have seen biological fluid sample volumes reduced to an extent that conventional SPE formats are not always suitable, or provide considerable analyte dilution and extended evaporation times. When extracting from sample volumes of less than 100μl, it is important the elution volumes and the sample preparation format are fully compatible with the original sample volumes being processed.
- Versatile 96-well format for small volume biological fluids
- Optimised well geometry for 5 and 10mg sorbent mass
- 50µl elution volumes for minimal sample dilution and reduced evaporation times
- Populated plates and loose wells
- Compatible with vacuum and positive pressure
- Automation compatible
The new TELOS MicroPlate is optimised for the sample processing and extraction of small volume biological fluids. The internal diameter, frit design and sorbent allow biological fluids to be processed. The TELOS MicroPlate is a modular design, allowing for flexibility in sample numbers. Full or partially populated plates can be processed using vacuum or positive pressure. In addition, the well outlet design ensures good collection plate penetration, removing any possibility of well to well cross contamination.
Sorbents
The TELOS MicroPlate is available in 5 and 10mg sorbent masses, packed with five TELOS neo SPE Sorbents – PRP, PCX, WCX, PAX and WAX.
Well Design
The individual wells of the TELOS MicroPlate are designed to both allow maximum interaction between the analyte and sorbent and ensure elution volumes are kept to a minimum. This is particularly important for sample volumes of 100µl and less, and ensures minimal evaporation times prior to analysis by LC-MS.
Elution Volumes and Recoveries
The optimised well and frit design (see Figure 1) of the TELOS MicroPlate provide an overall bed volume of 10 – 15µl. A well packed with 5mg sorbent allows analytes to be eluted in as little as 50µl.
Populated Plates and Loose Wells
The TELOS MicroPlate is available as either populated plates or loose wells. This allows the product to be used for both high throughput assays (populated plate) and assays where the sample numbers vary, populating the base plate with the required number of wells. When using vacuum, the unrequired ports on the base plate can be sealed.
The individual wells can be removed or added very easily, with a secure “click” when the wells are added to the base plate.
Vacuum and Positive Pressure
The TELOS MicroPlate is compatible with both vacuum and positive pressure manifolds. The well outlets extend beyond the plate “shelf” and ensure they fully penetrate the collection plate.
Recovery & Elution Volumes
The TELOS MicroPlate 5mg plate allows elution volumes of 50µl. The following recovery data and elution volumes are for two β-blockers, Metoprolol and Propranolol, when extracted from human plasma (50µl) using a TELOS neo PRP 5mg MicroPlate.