F.SIGHT - Model OMICS -Single-Cell Dispensing Into PCR Plates

• Precise Dispensing Enables Downstream Miniaturization
• Automated offset correction (AOC) adjusts droplet trajectory.
• Precise and reproducible cell deposition into PCR plates.
• Enables downstream miniaturization with lysis buffer volumes less than 1 μL.

• Gentle dispensing
• Higher cell viability
• EASY.ON magnetic holder
• User-friendly handling
• Faster setup time
• Cartridges with improved coating
• For a broad variety of samples
• Low cartridge-to-cartridge variation

• Gentle alignment of cells in the center of the dispensing cartridge for better cell detection
• Increases processing speeds and cell recovery
• Significantly improves handling rare cells

• AOC adjusts droplet trajectory
• Ensuring precise and reproducible cell deposition at the center of each well (96/384)
• Enabling downstream miniaturization of cell lysis in sub-microliter volumes (< 1 µl)
• Comprehensive documentation: images available for every AOC measurement documenting the offset

• Time lapse of single-cell deposition
• Clonality probability ~99%
• Nozzle images available for every well documenting dispensation of single cells
• Comprehensive documentation

Time

• System boot until experiment starts: <5 min
• Dispensing (96-well plate) single cells: ~2 min
• Dispensing (384-well plate) single cells: ~8 min
• Shutdown and cleaning time: <5 min

Bright-field and fluorescence

• Innovative dual-camera system
• Simultaneous capture of bright-field and fluorescence images at full resolution
• Control both cameras independently and freely adjust the power setting for the built-in blue laser
• Image-based cell detection

Fast and gentle single cell dispensing

• Low impact on cell viability/integrity
• Single cells are gently dispensed into a 96-well plate in less than 2 minutes
• or into a 384-well plate in less than 7 minutes
• Only 5-80 µl sample volume required

EASY.ON cartridges

• Exclusive cartridge is magnetically mounted for quick and easy loading
• Engineered by microfluidic experts to ensure cell viability with the gentlest handling
• Its single-use design eliminates the risk of cross contamination between samples

Compatible with robotic automation workflows

• F.SIGHT Omics’ lid opens automatically, making the fastest single-cell dispenser fully compatible with robotic automation workflows

Intuitive software

• Intuitively designed and fast
• Individual fluorescence experiments can be set up within minutes
• Get separate full-resolution bright-field and fluorescence imaging along with an overlay image

Cell focusing technology

• Gently aligns cells at the center of the dispensing cartridge for superior cell detection
• Cell morphology is captured and measured
• Increased processing speed and minimal cell loss; significant improvements when working with rare cell types