- Home
- Companies
- BellBrook Labs
- Products
- Transcreener - GDP Assay Kit - FP ...
Transcreener - GDP Assay Kit - FP Readout
The Transcreener GDP Assay with FP readout measures GDP via antibodies selective to GDP and a far-red fluorescent tracer. GDP produced in the reaction competes with the tracer, decreasing fluorescent properties and providing a fluorescent readout. The universal detection method allows screening of virtually any kinase in a high throughput format. The Transcreener Assay offers reagent stability and compatibility with commonly used multimode plate readers. The FP GDP Assay accommodates GTP concentrations ranging from 1 µM to 1,000 µM with excellent data quality (Z` ≥ 0.7) at low substrate conversion (typically 10-30%). Used in over millions of wells by pharma since 2006, Transcreener is the most extensively validated GDP detection method available backed by the best technical support in the industry.
- Direct Detection of unlabeled GDP
- Robust Assay Z` > 0.7 under initial velocity conditions
- Far-red fluorescent readouts minimize compound interference
- A safe, non-radioactive method
- Use with GTP concentrations from 1 µM to 1,000 µM
- Stable Assay reagents - minimum 8-hour reagent and signal stability even at room temperature!
- Measure enzymatic activity of GTPases
- Screen compound libraries for GTPase Modulators
- Quantify Inhibitor Potency
- Inhibitor Selectivity Profiling
- Measure Drug-Target Residence Time
Run your enzyme reaction, add Transcreener reagents, and read your plates. The GDP assay is compatible with 96, 384, and 1536-well formats.
Detection of GDP Released by KRAS (FP Readout)
Below is an example of the Transcreener GDP Assay with FP readout detecting GDP released by the GTPase, KRAS. The assay demonstrates linearity when raw data is converted to GDP using a standard curve.
Validation Criteria for Plate Readers
- 384-Well Format
- Z`-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
- Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
- Z` and Δ mP specifications to be met using Transcreener ADP2 Assay Reagents
- Read time to achieve Z` and Δ mP specifications ≤ 5 minutes
- Look for these stickers on instruments that have successfully met the validation criteria.
The instrument validation program is a collaborative effort with the major suppliers of multimode readers to insure that Transcreener assays perform optimally on your specific instrument. Scientists at Bellbrook and/or instrument vendor have tested Transcreener reagents with each instrument to determine optimal filters and settings for maximal assay performance. We provide you with application notes with all the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.