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ANTEC GmbH Analysen- und Prozesstechnik
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ANTEC - Model PrepLinc 2 2D -Gel-Permeation Chromatography System for High Lipid Content Samples

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ANTEC GmbH introduces the PrepLinc 2™ 2D Gel-Permeation Chromatography (GPC) system, specifically designed for the separation and purification of large molecules, especially fats and oils. Traditional GPC systems often struggle with high lipid content samples, requiring multiple runs to achieve the desired level of purification. The PrepLinc 2D GPC system allows for the injection of up to five grams of lipid matrix in a single run, significantly reducing processing time and solvent usage while increasing sample throughput. This system employs a two-column setup, enabling the processing of a larger sample volume and achieving optimal separation in a single injection. This innovation addresses the limitations of conventional GPC columns, which typically handle only one gram of matrix per run. The 2D GPC system is easily upgradable for existing PrepLinc systems and is optionally available for new installations. It has proven effective in applications such as dioxin analysis, where multiple GPC runs are standard practice, thus saving time and resources. For detailed application notes and technical support, ANTEC GmbH offers comprehensive service and consultation.
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GPC is used for extract purification to separate large molecules, especially fats and oils.
With 2D GPC, up to five grams of fat or matrix can be added to the GPC system with one injection.
This significantly reduces the running time of the GPC and increases the sample capacity with reduced solvent consumption.

 

For decades, gel permeation chromatography has held the leading position as the method of sample purification for residue analysis in terms of cleanliness of the eluates and low personnel expenditure for implementation.

However, even though the sensitivity and thus the detection limit of the analyzers used below have improved significantly in recent years, there are borderline cases in which the maximum amount of matrix of one gram used in the "normal" GPC column filled with 50 g of Bio-Beads S-X3 is not sufficient to reliably reach the required detection limit. Multiple runs with the GPC, with a maximum of one gram of matrix each, reduce the detection limits accordingly - but increase the running times per sample.
For example, in dioxin analysis it is common to carry out a quadruple run with GPC in order to then collect the eluates and analyze them further together. However, this means that the GPC system runs four times as long, with reduced capacity and increased solvent consumption.

We therefore asked ourselves how we could reduce the runtime and solvent consumption with reasonable technical effort so that GPC can continue to maintain its leading position. The development of 2D GPC is the answer: Up to 5 grams of matrix can be applied and processed in one injection. This means significant time and cost savings for our users.

 

The PrepLinc 2D-GPC method significantly increases the amount of sample applied to the GPC column combination with one injection. The typical matrix loading for conventional GPC columns is no more than 1 g of matrix per 5 ml injection volume. Conventional GPC methods may require multiple GPC runs to fully purify samples with high matrix content. The PrepLinc two-column gel permeation chromatography allows the sample to be processed through two GPC columns in series, increasing the matrix capacity by 5 times with minimal increase in run time compared to a regular injection. With 2D-GPC, maximum separation can be achieved in one sample injection.