Montana Molecular - Green and Red Fluorescent Cell Stress Assay

The Green and Red Fluorescent Cell Stress Assay provides a robust method for detecting stress responses in living cells. This assay involves the use of a bright green fluorescent protein that is activated under endoplasmic reticulum (ER) stress conditions or during the unfolded protein response (UPR). Utilizing fluorescence plate readers or imaging systems, these assays enable precise measurement of cellular responses to chemical or genetic stress. The technology enables high-throughput screening for compounds that modulate ER stress pathways, providing insights into mechanisms of neurodegeneration and protein misfolding diseases. Additionally, a ratiometric biosensor variant offers enhanced detection capabilities by measuring both green and red fluorescent signals. This dual color assay is versatile for applications in diverse cell types, including primary cells and induced pluripotent stem cell-derived cells. Packaged in a BacMam vector, it ensures efficient expression and compatibility with imaging systems, contributing to advanced research in cellular stress physiology.