Biotools, B & M Labs, S.A

BiotoolsModel 1U/µl -High-Fidelity DNA Polymerase

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High-fidelity DNA Polymerase from Pyrococcus furiosus, cloned in E.coli. with proofreading (3`-5` exonuclease) activity and a reduced error rate (10-fold lower than standard Taq DNA Polymerases).

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High-fidelity DNA Polymerase from Pyrococcus furiosus, cloned in E.coli. with proofreading (3`-5` exonuclease) activity and a reduced error rate (10-fold lower than standard Taq DNA Polymerases). 

Biotools Pfu DNA Polymerase generates blunt-ended amplification products, enabling their cloning into blunt-ended vectors. 

It is suitable for applications including:

  • Cloning 
  • Mutagenesis
  • Achieving greater fidelity than with Taq DNA polymerase
  • Amplifying fragments of up to 5 kb

Contents:

Biotools Pfu DNA Polymerase is supplied with the choice of 10 X Standard Reaction Buffer including 2 mM MgCl2 or a reaction buffer without MgCl2 (10 X Reaction Buffer MgCl2 Free) plus a separate tube of MgCl2 for optimising the magnesium concentration.

Make a question about Pfu DNA Polymerase>>

  • Polymerase Pfu
  • Hot Start effect No
  • Fidelity (vs Taq) ++++
  • Concentration 1 U/μl
  • GC-Rich PCR Performance No
  • Reaction Format Separate components
  • Amplification size Up to 5 kb
  • Product size 100 U, 250 U
  • Exonuclease Activity 3’ - 5’
  • Shipping condition Cold shipping
  • Storage condition -20ºC
  • Regulatory Statement For research use only. Not for use in diagnostic procedures