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HOT FIREPol - Hot-start PCR Mix for GC-rich Templates
FromSolis BioDyne
HOT FIREPol GC Master Mix has been developed for working with difficult GC-rich templates and DNA secondary structures. It shows excellent amplification with templates up to 79% GC content. The master mix contains hot-start Taq polymerase HOT FIREPol®, MgCl2, dNTPs, and a special buffer for high yield GC-rich amplification. Extra vials of 100% DMSO and 25 mM MgCl2 enable flexibility in reaction optimization.
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- excellent amplification with templates up to 79% GC content
- suitable for templates up to 5 kb
- suitable for fragment analysis
- Concentration: 5x
- Hot-start: yes, initial activation in 12-15 min.
- Ready to load: no
- Fidelity: 1 x Taq
- Cloning Type: T/A cloning
- Amplicon Size: up to 5 kb
- Difficult templates: robust on GC-rich templates
- HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
- 5x HOT FIREPol® GC Buffer with 7.5 mM MgCl2: 1x PCR solution –1.5 mM MgCl2
- dNTPs: dATP, dCTP, dGTP and dTTP
- Bovine serum albumin (BSA) to enhance PCR reaction
- In separate vials:
- 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.
- 25 mM MgCl2 for further optimization