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  5. HOT FIREPol - Probe Universal qPCR Mix

HOT FIREPol - Probe Universal qPCR Mix

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HOT FIREPol® Probe Universal qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes. Proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets. HOT FIREPol® Probe Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the 5` flap endonuclease activity. The Mix contains internal passive reference dye that is compatible with most qPCR cyclers including high ROX or low ROX reference signal requiring platforms. The Mix contains dUTPs to prevent cross-contamination with UNG treatment.

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Precisely-optimized real time qPCR master mix for probe-based assays. This master mix has been developed for TaqMan® probes but is also suitable for other hydrolysis probe types.

  • suitable for singleplex and duplex assays
  • high specificity and sensitivity
  • one qPCR mix for all cyclers (except capillary)
  • superior results with templates with up to 75% GC content
  • contains dUTP to prevent cross-contamination when used with UNG
  • Benchtop stability for 48 hours for preassembled reactions

  • DNA/LNA hydrolysis probe-based assays
  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination

  • Concentration: 5x
  • Hot-start: yes, initial activation in 10 min
  • Detection type: Probe-based
  • Reference dye: reference dye based on ROX
  • Compatible real-time instruments: Most qPCR platforms. Check Your cycler!

  • HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
  • 5x Probe Universal qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3 mM MgCl2
  • dNTPs including dUTP:  Mix allows UNG treatment to prevent carryover contamination from previous runs.
  • IMPORTANT: UNG is not included in the HOT FIREPol® Probe Universal qPCR Mix
  • Reference dye based on ROX: For multiplex application - if ROX dye is used as one of the fluorophores, the internal reference might interfere with the signal.
  • In separate vial
  • 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.