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HOT FIREPol SolisGreen - Highly Sensitive Dye-Based qPCR Mix

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Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye. It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.





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Optimized ready-to-use solution for dye-based real-time quantitative PCR assays. SolisGreen® dye is characterized by high sensitivity and
great PCR efficiency with low template amounts for accurate and reproducible results

  • improved detection of low target concentrations
  • higher fluorescence level
  • reaction set-up and shipment without dry ice
  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination

  • Concentration: 5x
  • Hot-start: yes, initial activation in 10 min
  • Detection type: dye-based, includes SolisGreen® intercalating dye
  • Reference dye: based on ROX
  • Compatible real-time instruments: Most qPCR cyclers except platforms that require high ROX (HOT FIREPol® SolisGreen qPCR Mix is not compatible with the Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems.) Check Your cycler!
  • HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
  • 5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
  • dNTPs: dATP, dCTP, dGTP and dTTP
  • SolisGreen® dye
  • Internal reference based on ROX dye


Properties

Concentration: 5x

Hot-start: yes, initial activation in 10 min

Detection type: dye-based, includes SolisGreen® intercalating dye

Reference dye: based on ROX

Compatible real-time instruments: Most qPCR cyclers except platforms that require high ROX (HOT FIREPol® SolisGreen qPCR Mix is not compatible with the Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems.) Check Your cycler!

Mix Components

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start

5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2

dNTPs: dATP, dCTP, dGTP and dTTP

SolisGreen® dye

Internal reference based on ROX dye

SolisGreen® dye

This dye-based qPCR mix contains next-generation DNA binding dye  SolisGreen®. The fluorescence spectra of this dye is similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. SolisGreen® dye shows:

  • high fluorescence level
  • high sensitivity for detecting low template concentrations
  • high stability for room temperature storage