Hygromycin B Gold Solution
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.It has been reported to interfere with translocation [1] and to cause mistranslation at the 70S ribosome.
Hygromycin B is used as a selective agent in molecular genetics experiments on a wide variety of eukaryotic and prokaryotic species.
The hph gene confers hygromycin-resistance to cells expressing it and many vectors carrying the hphgene are available within the scientific community.
Typically, mammalian cells are sensitive to Hygromycin B concentrations of 50-200 µg/ml, and bacteria to 50-100 µg/ml.
Product concentration:100 mg/ml solution
CAS number: 31282-04-9
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 90% (HPLC)
Endotoxin level: < 0.5 EU/mg
Physicochemical characterization: pH, appearance
Cell culture tested: potency validated in hygromycin‑sensitive and hygromycin-resistant mammalian cell lines
Non‑cytotoxicity of trace contaminants: absence of long‑term effects confirmed in hygromycin‑resistant cells
Hygromycin B Gold (previously named HygroGold™) is supplied as a sterile filtered yellow solution at 100 mg/ml in HEPES buffer.
This product is available in two pack sizes:
- ant-hg-1: 10 x 1 ml (1 g)
- ant-hg-5: 1 x 50 ml (5 g)
Hygromycin B Gold is shipped at room temperature.
Upon receipt, it should be stored at 4°C or -20˚C.
The working concentrations of Hygromycin B Gold for mammalian cell lines vary from 50 to 200 μg/ml, in a few cases up to 500 μg/ml.If you notice unusually high cytotoxicity when switching to Hygromycin B Gold, you may need to adjust your working concentrations.Suggested concentrations of Hygromycin B Gold for selection in some examples of mammalian cells are listed.
