PrinCE - LED and Laser Induced Fluorescence Detectors

For fluorescent compounds, concentration limits of detection down to 10-12 mol/l have been reported. Bearing in mind that typically about 10 nl of sample is injected, this equals about 10-20 mol! LIF detection can be more sensitive than any other commercially available detector, even if the compound is not fluorescent itself. Of course a suitable label should be available.

Comparable with the LIF, LED is from 10–100 times greater than conventional fluorescence and up to 100,000 times more sensitive than UV detection. Low noise and interference molecules will be detected. Only labeled or native fluorescence can be measured.

Specifications — LIF detector

• Available Laser Wavelengths 266, 325, 355, 410, 442, 473, 488, 514, 532, 594, 633, 638, 642 & 780 nm.
• Emission Filter: The emission filters depend on the wavelength and generally contain highpass filters
• LIF Capillaries OD 365 μm; ID from 20 to 320 μm
• Cell Detection Volume The cell is a small part of the capillary and depends on the capillary ID, for example 0.4 nL with a 50 μm ID.
• Signal Outputs - External Ethernet for Network communications. - External Event (detection of a relay state) function. Start/stop command port for an external event command (relay opened or closed). - Analog output: Processed: 0-1V(DC) for range 0-50 RFU. 0-100mV(DC) for range 0-5 RFU.
• Power Requirements 100-240 VAC, 47/63 Hz, 1.5 A
• Dimensions and Weight 43.0 cm / 16.9”(H) x 23.0 cm / 9.1”(W) x 34.0 cm / 13.33” (D). 12 kg / 26.4 lbs

Specifications — LED detector

• Available LED Wavelengths 450, 480, 530 nm
• Emission Filter: The emission filters depend on the wavelength and generally contain highpass filters
• LIF Capillaries OD 365 μm; ID from 20 to 320 μm
• Cell Ddetection Volume The cell is a small part of the capillary and depends on the capillary ID, for example 0.4 nL with a 50 μm ID.
• Signal Outputs - External Ethernet for Network communications. - External Event (detection of a relay state) function. Start/stop command port for an external event command (relay opened or closed). - Analog output: Processed: 0-1V(DC) for range 0-50 RFU. 0-100mV(DC) for range 0-5 RFU.
• Power Requirements 100-240 VAC, 47/63 Hz, 1.5 A
• Dimensions and Weight 43.0 cm / 16.9”(H) x 23.0 cm / 9.1”(W) x 34.0 cm / 13.33” (D). 12 kg / 26.4 lbs