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Lumigen - Model APS-5 -Chemiluminescent Substrate for ELISA Detection
FromLumigen, Inc.
Lumigen APS-5 is a proprietary acridan based chemiluminescent substrate for ELISA detection of alkaline phosphatase (AP) conjugated molecules. The unique acridan chemistry of Lumigen APS-5 provides temperature insensitive light output and more rapid peak intensity as compared to the dioxetane based AP substrates, Lumi-Phos 530 and Lumi-Phos Plus.
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- Excellent sensitivity - low picogram to femtogram
- Rapid peak intensity - reduced assay time and increased throughput
- Temperature insensitive - analytical results are insensitive to temperatures from 22° C - 35° C reducing the need for precise temperature control
- Sustained luminescence - timing is less critical with light intensities able to be read at any time to produce linear calibration curves
The light-output peak intensity of Lumigen APS-5 reaches sustained maximum within seconds of substrate addition. Light emission is maximal at 450 nm.
- Enzyme : Alkaline Phosphatase (AP)
- Application : ELISA
- Sensitivity : Low picogram to femtogram
- Storage Conditions : 2-8° C; Store in amber bottle to protect from light
- Stability : 1 year stored at 2-8° C
- Working Solution : Ready to use solution