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Marianas LightSheet - Versatile Multiview Light Sheet Microscopy System
The Marianas LightSheet system integrates dual inverted selective plane illumination microscopy (diSPIM) with an advanced research-grade inverted microscope, allowing for extensive imaging capabilities tailored for model organisms. Known for its low phototoxicity and substantial sample space, this system supports diverse specimen types and experimental protocols such as photoablation and photomanipulation. It elevates experimental potential by incorporating spinning disk confocal imaging, total internal reflection fluorescence (TIRF), and fluorescence lifetime imaging microscopy (FLIM), all operated through the sophisticated SlideBook software. The flexible configuration features multiple high-end components like MEMs mirror scanners, optimized objective pairs, and motorized drives to enable precise manipulation and imaging. With its modularity, users can seamlessly integrate additional functionalities like laser ablation and photomanipulation, all optimized for intricate light sheet experiments and complex data processing through GPU acceleration.
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Marianas LightSheet (MLS) combines the low phototoxicity and large sample space of dual inverted selective plane illumination microscopy (diSPIM) with the power and flexibility of a research-grade inverted microscope system. MLS supports a wide variety of specimen types and sizes as well as experimental designs including photoablation, photomanipulation, and computer-generated holography. Add spinning disk confocal, TIRF and FLIM to transform the system into a versatile multimodal imaging system all controlled with SlideBook software.
Dual Inverted Selective Plane Microscopy
Dual inverted selective plane microscopy (diSPIM) uses dual camera and scanner objective pairs positioned at 45° to the specimen plane to alternate capture and excitation between Path 1 and Path 2. The flexible system geometry allows for single- and dual-sided imaging with conventional sample mounting. SlideBook seamlessly controls all hardware modalities, deskewing, stitching/montaging, Multiview Reconstruction and joint deconvolution resulting in isotropic sub-cellular resolution across a wide range of samples. Marianas LightSheet integrates diSPIM with a research-grade inverted microscope enabling complex multi-modal light sheet experiments with photoablation, photostimulation and computer-generated holography.
SlideBook Multiview Reconstruction
SlideBook for MLS supports several methods to deskew, rotate, register and deconvolve diSPIM images. Dual-view capture registration is performed either through Point of Interest Detection or 3i’s proprietary Local Cross-Correlation and Registration algorithms. Results are instantly displayed in 3D for quick adjustment to achieve an ideal fit before joint deconvolution produces a fused image. Large datasets that would otherwise require high-powered computer resources can be automatically split into a computer’s available RAM and computed in parallel utilizing GPU-optimized processes.