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Model Agilent -Protein and Peptide Sample Preparation Automated

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The challenge: How do you bring further standardization, reproducibility and hands on time savings to proteomics sample preparation for  LC-MS analysis when you have already successfully addressed  the many challenges of time, sample contamination, sample loss and reproducibility? The solution: In conjunction with Agilent, PreOmicsĀ®` kits are suitable for use on the Agilent Bravo platform. Meeting the demands of reproducible, robust and sensitive proteomics sample preparation for LC-MS analysis, these applications allow research labs to partially automate proteomics processing, decreasing the hands on time.
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1. Adding samples

Samples should be liquid, homogenates or a soft pellet.

2. iST kit setup

Add the appropriate iST kit components to Agilent Bravo.

3. Sample preparation

Protocols need to be programmed by an in-house specialist.

4. MS analysis

Inject samples into your LC-MS system.

5. Data analysis

Process your data to see improved peptide identities.

The partially automated Agilent Bravo requires off-board centrifugation. The figures above show data generated on the Agilent Bravo in a partially-automated protocol, using 1 µL plasma with analysis on a Thermo Q-Exactive HF Orbitrap.

Smile
Improved sample quality
  • High reproducibility R2=0.9
  • Working range: 1-100 µg
  • Increased peptide and protein identifications
Relax
Sample preparation automated
  • Reduces manual processing steps
  • Generates standardized and highly reproducible proteomics LC-MS results
  • Applications for the PreOmicsĀ® protocols
Realize
Cleaner peptides ready for analysis
  • Dual clean up step removes hydrophilic and hydrophobic contaminants
  • Achieve ready-to-measure peptides
  • Reduce mass spectrometry downtime