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Model GIST-RT44 -Mutation Detection Kit
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the GI tract. Over 80% of GISTs harbor activating mutation in c-KIT and approximately 5-7% in PDGFRA. Although these mutations cause tumorigenesis, they also predict response to targeted therapies and provide prognostic information.
EntroGen’s GIST mutation screening panel is a polymerase chain reaction (PCR)-based assay that uses allele-specific primers to identify the presence of somatic mutations in c-KIT and PDGFRA genes. The testing procedure involves three (3) simple steps:
- Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), or fresh frozen tumors
- Amplification of regions of the c-KIT and PDGFRA genes using allele-specific primers
- Analysis of the captured amplification data.
This test can be completed in approximately 2 hours from isolation of DNA to test result.
EntroGen’s GIST mutation screening panel requires a real-time PCR instrument capable of detecting FAM, VIC, ROX and CY5 fluorescent probes.
This test includes reagents required for the PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation are not included.
USA: Available forresearch use only (RUO). Not for use in diagnostic procedures.
Europe: Available for research (RUO) and diagnostic (CE-IVD) purposes.