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Model iST -Fast, Reliable, Standardized Methods for Sample Preparation
FromPreomics Gmbh
Fast, reliable, standardized methods for sample preparation ready for mass spectrometry analysis. The challenge: Complex, multi-step methods of proteomics sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss. Preparing labelled samples over an extended time period with a complex method leads to variation in results. This challenge is exacerbated with large numbers of samples and small starting amount of input material. The solution: PreOmics® improved sample technology enables robust and reproducible sample preparation. The iST method provides significant time savings as complex workflows previously requiring many different reagents taking 48 hours are replaced by a single kit containing all standardized reagents. The iST kit allows proteomics sample preparation for mass spectrometry analysis, whether manually, semi or fully automated on the PreON, in a 2 hour time window.
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1. Sample
Samples should be liquid, homogenates or a soft pellet.
2. Lyse, reduce, alkylate
Add LYSE reagent, and incubate for 10 mins at 95°C.
3. Digest
Cool, add DIGEST reagent, and incubate for 60 mins at 37°C.
4. Purify
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyze to see improved peptide identities.
While standard proteomics sample preparation takes around 44 hours – the same time as flying around the world non-stop, PreOmics® kits take the same time as flying from our Munich HQ to London or from Boston to Chicago. Increase your sample throughput, improve quality and produce reliable data. It’s as easy as i-S-T!
Smile
Improved sample quality
- High reproducibility: R2>0.9
- Working range: 1-100 µg
- Increased peptide and protein identifications
- Shipped at RT
Relax
Quicker methodology
- 1 hour hands-on time, 2 hours total time
- Single step incorporating lysis, reduction and alkylation
- All-in-one solution, all reagents provided
- Compatible with standard lab equipment
Realize
Cleaner peptides ready for analysis
- Dual clean up step removes hydrophilic and hydrophobic contaminants
- Achieve ready to measure peptides
- Reduce mass spectrometry downtime
- Manual to fully automated processing options available
