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Nicoya OpenSPR - Nanotechnology Enabled Sensors for Surface Plasmon Resonance
OpenSPR Sensors, developed by Nicoya Lifesciences, are designed to provide a cost-effective solution for surface plasmon resonance (SPR) applications. These sensors harness advanced nanotechnology for manufacturing, which results in a significant decrease in expense compared to traditional SPR sensors, while maintaining exceptional quality standards that ensure reliable and repeatable measurements. With a coefficient of variation (CV) of less than 5% across critical optical properties, these sensors offer stability in diverse environments, accommodating a range of solvents, buffers, and reagents. Notably compatible with both OpenSPR and OpenSPR-XT instruments, OpenSPR Sensors enable researchers to conduct numerous experiments economically. The High Sensitivity Sensors variant enhances localized sensitivity, proving advantageous for small molecule analysis and improving signals of larger biomolecules, thus extending the applications of SPR technology.
OpenSPR™ is a powerful instrument providing in-depth label-free binding kinetics for a variety of different molecular interactions. One of the most common applications of surface plasmon resonance is the analysis and quantification of the interactions between proteins. In this application note, OpenSPR™ is used to analyze the kon , koff , and KD of two different protein ligands (M1 and M2) to one protein analyte. The biomolecular system and conditions were developed by Dr. Olan Dolezal from CSIRO.
Materials and Equipment
- OpenSPR Instrument
- OpenSPR Streptavidin Sensor Chip
- TraceDrawer Kinetic Analysis Software
- Biotinylated Ligand Protein M1 and M2 (27 kDa each)
- Analyte protein A1 (50 kDa)
- Running buffer (HBS, 0.005% Tween20, 0.1 mg/ml BSA)
- Regeneration buffer (HCl pH 2.0)
